抗重金属细菌BCD1的分子鉴定及系统发育分析  

Molecular Identification and Phylogenetic Analysis of Heavy Metal-Resistant Bacreria BCD1

作　　者：乔宏萍[1] 裴梓汝 贺超 李闯 许嘉 张玉荣 崔剑越 王青[1] QIAO Hongping;PEI Ziru;HE Chao;LI Chuang;XU Jia;ZHANG Yurong;CUI Jianyue;WANG Qing(Department of Biology,Taiyuan Normal University,Jinzhong 030619,China)

机构地区：[1]太原师范学院生物系,山西晋中030619

出　　处：《太原师范学院学报（自然科学版）》2018年第2期90-92,96,共4页Journal of Taiyuan Normal University:Natural Science Edition

基　　金：太原师范学院大学生创新项目(XCY1626)

摘　　要：BCD1是一株高度耐受重金属镉的细菌,本研究对其进行形态学、生理生化特性和16SrDNA序列鉴定.结果发现,细菌BCD1革兰氏染色为阴性,细胞呈短杆菌,单个存在,好氧.通过16SrRNA测序Blast比对后发现BCD1和恶臭假单胞菌(Pseudomonas putida)相似性为100%,聚类分析后二者聚为一个分支,由此可以确定菌株BCD1为恶臭假单胞菌(P.putida).To study the strain BCD1 which was a cadmium resistance bacteria was identified by morphological observation,physiological-biochemical experiments and sequence analysis.The results showed that the bacteria BCD1 were gram negative,and the cells were short bacilli,single and aerobic.After 16 SrRNA sequencing and BLAST comparison,we found that the similarity between BCD1 and Pseudomonas putida was 100%.So the strain BCD1 was identified as Pseudomonas putida by phylogenetic analysis.

关 键 词：抗重金属 生理生化特性 16SrDNA 系统发育树 

分 类 号：G424[文化科学—课程与教学论]