植物激素对微拟球藻油脂及二十碳五烯酸积累的影响  

Effects of plant hormones on the lipid and eicosapentaenoic acid production in Nannochloropsis

作　　者：李悦明 崔球[2] 徐建春 宋晓金[2] 夏修峦 李霞 范栋[3] 李妍 LI Yueming;CUI Qiu;XU Jianchun;SONG Xiaojin;XIA Xiuluan;LI Xia;FAN Dong;LI Yan(Qingdao Langyatai Group Co.,Ltd.,Qingdao 266400,China;Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China;Huangdao Entry Exit Inspection and Quarantine Bureau,Qingdao 266400,China;Huangdao Drug Supervision and Administration Bureau,Qingdao 266400,China)

机构地区：[1]青岛琅琊台集团股份有限公司,山东青岛266400 [2]中国科学院青岛生物能源与过程研究所,山东青岛266101 [3]黄岛出入境检验检疫局,山东青岛266400 [4]青岛市黄岛区食品药品监督管理局,山东青岛266400

出　　处：《发酵科技通讯》2018年第2期82-87,共6页Bulletin of Fermentation Science and Technology

基　　金：工信部绿色制造系统集成项目(Z135060009002);青岛市自主创新重大专项(16-7-1-11-zdzx-nsh)

摘　　要：考察了6种植物激素对微拟球藻油脂积累与EPA产量的影响,并初步探讨了其对油脂合成的调控机制.每种植物激素对微拟球藻油脂积累都有促进效果.其中添加20mg/L IBA时油脂产量提高最多,油脂质量分数提高20.94%.其次是添加15mg/L的BA,油脂质量分数提高20.34%.同时,BA在质量浓度为15mg/L时,EPA质量分数提高了29.34%.实时定量PCR证实BA等激素能显著提高微拟球藻中油脂合成相关基因的转录水平.证实了植物激素可用于提高微拟球藻EPA的产量,为利用植物激素提高微拟球藻发酵生产EPA提供了实验依据.The effects of six plant hormones on the lipid and EPA production of Nannochloropsis oculata M44 were investigated.Each plant hormone has a beneficial effect on lipid accumulation.When 20 mg/L IBA was added,the maximum lipid production was achieved with an increase of20.94% compared to the control group.The EPA and lipid production were increased by 29.34%and 20.34% at a concentration of 15 mg/L BA.Real-time quantitative PCR confirmed that BA and other hormones can significantly increase the transcriptional level of lipid synthesis-related genes in Nannochloropsis oculata M44.This study confirms that plant hormones can be used to increase the EPA production of Nannochloropsisoculata M44,which provides the experimental basis for the production of EPA.

关 键 词：二十碳五烯酸 植物激素 油脂合成 微拟球藻 实时定量PCR 

分 类 号：Q815[生物学—生物工程]