流加补料技术在大观霉素发酵中的应用  

Application of continuous fed-batch cultivation in spectinomycin fermentation

作　　者：方声 赖月琴 陈斌 陈小辉 徐铮 FANG Sheng;LAI Yueqing;CHEN Bin;CHEN Xiaohui;XU Zheng(Zhejiang Jinhua CONBA Bio Phann Co.,Ltd.,Jinhua 321016,China)

机构地区：[1]浙江金华康恩贝生物制药有限公司,浙江金华321016

出　　处：《发酵科技通讯》2018年第2期92-96,共5页Bulletin of Fermentation Science and Technology

摘　　要：为稳定壮观链霉菌在发酵后期的代谢环境和大观霉素生物合成速率,提高大观霉素发酵生产水平,研究发酵过程连续流加糊精、硫酸铵,氨水调节发酵液pH,发酵过程变温培养,延长发酵周期至168h,发酵过程发酵液放料3次,以流加补料新工艺替代传统的间歇补加全料工艺,大观霉素发酵单位平均提高74%,产量平均提高111%.This study aims to stabilize the metabolic environment and biosynthetic rate of spectinomycin in the late stage of Streptomyces spectabilis fermentation.Dextrin and ammonium sulfate were continuously fed,and pH of the fermentation broth was controlled by ammonia solution.The fermentation period was prolonged to 168 h,and the fermentation broth was withdrawn 3 times during the process.When the traditional fed-batch cultivation was replaced by the continuous one,the titer and yield of spectinomycin were increased by 74% and 111%,respectively.

关 键 词：大观霉素 流加补料 间歇补料 壮观链霉菌 

分 类 号：TQ465.9[化学工程—制药化工]