磷脂酶产生菌株的筛选·鉴定和培养条件优化  

Screen and Identifacion of Phospholipase-producing Strain and Optimization of Its Culture Conditionsits

作　　者：朱丹峰 胡雪芹 张洪斌 ZHU Dan-feng;HU Xue-qin;ZHANG Hong-bin(School of Biological and Medical Engineering,Hefei Univesity of Technology,Helei Anhui 23000)

机构地区：[1]合肥工业大学生物与医学工程学院,安徽合肥230009

出　　处：《安徽农业科学》2018年第19期9-12,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]筛选具有磷脂酶活力的菌株。[方法]采用磷脂平板初筛和摇瓶复筛筛选菌株,采用单因素试验研究菌株发酵产酶的最佳条件。[结果]最佳发酵配方和培养条件:复合碳源麦芽糖与大豆卵磷脂比例为3∶1(1.5%∶0.5%),最佳氮源为酵母粉1.0%,无机盐组成为NaH_2PO_4·3H_2O 0.05%,K_2HPO_4·2H_2O 0.10%,MgSO_4·7H_2O 0.06%,CaCl_2 0.05%,发酵温度30℃,发酵时间4 d,比初筛时提高1.61倍。菌株所产磷脂酶是低温酶,其最适作用温度是30℃,最适作用pH为7.5。[结论]该研究为菌株产酶及酶的进一步研究提供参考。[Objective]To screen a strain of phospholipase-activity. [Method]Strains were screened by phospholipid plate screening and shake flask screening,and single factor experiments were carried out to study the effect on the enzyme production by the strain.[Result]Best fermentation condition： the best compound carbon sources was the ratio of maltose and lecithin of 3 ∶1,the best initrogen source was yeast powder of 1. 0%,the best salt combination was NaH2PO4·3H2O 0.05%,K2HPO4·2H2O 0.10%,MgSO4·7H2O 0.06%,CaCl2 0. 05%,its optimum enzymeproducing temperature and time are 30 ℃ and 4 day resperctively,which was 1. 61 times higher than preliminary screeen. It could not tolerate high temperature,its optimum reaction temperature and pH are 30 ℃ and 7. 5,resperctively.[Conclusion]The results can be regarded as the reference for further research on fermentation and enzymatic application.

关 键 词：磷脂酶 菌株筛选 鉴定 产酶优化 

分 类 号：S3[农业科学—农艺学]