猪脂肪沉积和胚胎附植期FTO基因的表达及碱基突变检测  

Detection of FTO expression and SNPs during fat deposition and embryo implantation in pigs

作　　者：付言峰[1] 李兰[2] Robert V.Knox 李碧侠[1] 方晓敏[1] 任守文[1] FU Yan-feng;LI Lan;ROBER V.Knox;LI Bi-xia;FANG Xiao-min;REN Shou-wen(Institute of Animal Science/The Key Laboratory of Animal Breed Improvement and Reproductive,Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform,Nanjing 210014,China;Institute of Animal Immune Engineering,Jiangsu Academy of Agricultural Sciences,Nanjing 100193,China;Department of Animal Sciences,University of Illinois at Urbana-Champaign,Urbana,IL 61801,USA)

机构地区：[1]江苏省农业科学院畜牧研究所/动物品种改良和繁育重点实验室/江苏省农业种质资源保护与利用平台,江苏南京210014 [2]江苏省农业科学院动物免疫工程研究所,江苏南京100193 [3]美国伊利诺伊大学,伊利诺伊厄巴纳香槟61801

出　　处：《江苏农业学报》2018年第3期591-598,共8页Jiangsu Journal of Agricultural Sciences

基　　金：国家生猪现代产业技术体系南京综合试验站项目(CARS-36);国家科技支撑计划项目(2015BAD03B01-08);国家自然科学基金项目(31201767);江苏省农业科技自主创新基金项目[CX(17)2002];江苏省科技计划重点及面上项目(BE2017324)

摘　　要：为了研究脂肪肥胖相关基因(FTO)对猪脂肪沉积和胚胎附植的影响,本研究以太湖流域10头苏山猪和3头梅山猪母猪为试验对象,利用Real-time q PCR方法分析了该基因在脂肪沉积过程中苏山猪不同组织样和胚胎附植过程中梅山猪各组织样中的mRNA的表达,并用克隆测序方法检测了苏山猪群中FTO编码序列上的遗传变异。结果表明,脂肪沉积过程中,苏山猪16种组织样中均检测到FTO的mRNA表达,且背膘中的表达量极显著高于其他任何组织(P<0.01),高脂猪背最长肌中的FTO表达量显著高于低脂猪(P<0.05)。胚胎附植时期,梅山猪15种组织样中均检测到FTO的mRNA表达,且繁殖组织中的表达量显著高于其他组织(下丘脑除外)(P<0.05),表达量前2位组织样依次为:子宫、胚胎。苏山猪FTO编码序列(15~1 532 bp)上共发现10个c SNPs突变,其中有8个直接引起了编码氨基酸的变化。综上所述,FTO作用广泛,很可能在猪脂肪沉积和胚胎附植过程中发挥着重要的作用,其c SNPs可成为潜在的猪肉质性状和繁殖性状育种的分子标记。In order to study the effect of fat mass and obesity associated gene（ FTO） on fat deposition and embryo implantation in pigs,the m RNA expression of porcine FTO was analyzed by real-time quantitative PCR during fat deposition and embryo implantation in Sushan and Meishan pigs. Besides,the genetic variation in FTO coding sequences（ CDS） was investigated by cloning and sequencing in Sushan pigs. The results showed that during fat depostion,FTO m RNA was expressed in all 16 tissues of Sushan pigs,the expression level in backfat was significantly higher than that in any other tissue（ P0. 01）,and expression level in high-fat pigs was significantly higher than that in low-fat pigs（ P0. 05）. During embryo implantation,FTO m RNA was expressed in all 15 tissues of Meishan pigs,expression level in reproduction tissues was significantly higher than that in any other tissue（ P0. 05）,and the first two tissues were uterus and embryo. Ten c SNPs were found in FTO coding sequences（ 15-1 532 bp）,and eight c SNPs could directly cause changes in the encoded amino acid. In conclusion,FTO has a wide function,and it may play an important role in the regulation of fat deposition and embryo implantation,and c SNPs could become potential molecular markers for meat traits and reproduction traits breeding in pigs.

关 键 词：猪 FTO 脂肪沉积 胚胎附植 表达 

分 类 号：Q786[生物学—分子生物学]