微囊藻毒素和温度对萼花臂尾轮虫热激蛋白Hsp70水平的影响  

Effects of the microcystin (MC-LR) and temperature on the heat shock protein 70 (Hsp70) levels in the rotifer Brachionus calyciflorus

作　　者：徐秋磊 汪婵 陈莹莹 项贤领[1,2] 席贻龙 XU Qiulei;WANG Chan;CHEN Yingying;XIANG Xianling;XI Yilong(College of Life Sciences,Anhui Normal University,Wuhu 241000;Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province,Wuhu 241000)

机构地区：[1]安徽师范大学生命科学学院,芜湖241000 [2]安徽省高校生物环境与生态安全省级重点实验室,芜湖241000

出　　处：《环境科学学报》2018年第8期3339-3346,共8页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.31470015;31200324);安徽省自然科学基金(No.1708085MC79);重要生物资源保护和利用研究安徽省重点实验室专项基金~~

摘　　要：微囊藻毒素(MCs)是由有毒蓝藻(尤其是铜绿微囊藻)产生的一类藻毒素,可对水生生物和人类健康造成严重危害,然而目前有关浮游动物对MC-LR胁迫的应激响应研究较为少见.因此,本文探究了微囊藻毒素MC-LR和温度对采自青海西宁的萼花臂尾轮虫(Brachionus calyciflorus)热激蛋白Hsp70水平的影响.结果表明,温度、MC-LR及其交互作用对萼花臂尾轮虫Hsp70含量具有显著影响.在各温度条件下,MC-LR均诱导了轮虫Hsp70水平的上调;虽然Hsp70的表达水平在低剂量MC-LR处理下上调不显著,但在高剂量MC-LR(6 mg·L-1)处理下上调却极为显著;另外,以24℃处理为对照组,在20和28℃温度胁迫下各MC-LR处理组轮虫的Hsp70含量均显著增加,但16℃实验组与24℃对照组间未见显著差异,这可能与诱导轮虫Hsp70表达的环境温度阈值或机体对低温环境的长期适应性进化和调节机制有关.Microcystins（ MCs） produced by toxic cyanobacteria,especially the most dominant species Microcystis aeruginosa,are of particular concerns due to its serious threats to the health of aquatic lives and human beings. However,up to date,few investigations have been conducted on the responses of zooplanktons to MC-LR stress. In this study,we attempted to assess the effects of microcystin（ MC-LR） on the levels of heat shock protein Hsp70 at different temperatures in the rotifer Brachionus calyciflorus sampled from Xi＇ ning city,Qinghai province. The results show that the Hsp70 levels of the rotifer B. calyciflorus were significantly affected by MC-LR concentration,temperature and their interactions. All of the Hsp70 levels were shown to be upregulated as the MC-LR concentration increased under the four different temperatures（ 16,20,24 and 28 ℃）,and the up-regulation was especially significant at relatively high dosage of 6.0 mg·L-1 MC-LR; in addition,the Hsp70 were remarkably up-regulated at 20 ℃ and 28 ℃,compared to that of 24 ℃ as the control,regardless of the MC-LR concentration; however,no significant differences were detected between the Hsp70 levels at 16 ℃ and at 24 ℃,which is probably attributed to the ambient temperature threshold,long-term adaptive evolution to the local low temperature of the rotifer species in this study.

关 键 词：萼花臂尾轮虫 微囊藻毒素-LR 温度 热激蛋白Hsp70 WESTERN blot技术 

分 类 号：X171.5[环境科学与工程—环境科学]