ADAM12L在基底细胞样乳腺癌组织中的表达及其临床意义  

Expression of ADAM12L in the tissues of basal-like breast cancer and its clinical significance

作　　者：马波 谢尚闹[1] 周娟娣[1] 王晓红[2] MA Bo;XIE Shang-nao;ZHOU Juan-di(Department of Breast Surgery,Zhejiang Hospita)

机构地区：[1]浙江医院乳腺外科,浙江杭州310013 [2]湖州市中心医院乳腺外科,浙江湖州313000

出　　处：《中华全科医学》2018年第10期1626-1628,1705,共4页Chinese Journal of General Practice

基　　金：浙江省医药卫生科技计划项目(2018255510);浙江省自然科学基金一般研究项目(LY18H040010)

摘　　要：目的探讨ADAM12L在基底细胞样乳腺癌组织中的蛋白和核酸表达水平,并分析其临床意义。方法应用免疫组化法检测86例基底细胞样乳腺癌组织和20例良性乳腺纤维瘤组织的ADAM12L蛋白表达水平,并进一步采用实时荧光定量PCR方法检测60例基底细胞样乳腺癌组织及其配对癌旁组织的ADAM12L m RNA表达量。结果免疫组化染色显示,基底细胞样乳腺癌标本中ADAM12L蛋白阳性表达率(84.9%,73/86)显著高于良性纤维瘤组织样本中ADAM12L蛋白阳性表达率(45%,9/20),P<0.05;淋巴结转移阳性者的ADAM12L阳性率(73.8%,31/42)明显高于无淋巴结转移者(47.8%,21/44),χ~2=5.073,P=0.024;临床分期Ⅲ~Ⅳ者,ADAM12L阳性率(74.5%,41/55)明显高于临床分期Ⅰ~Ⅱ者(35.5%,11/31),χ~2=11.073,P<0.01。real-time PCR检测结果显示ADAM12L在基底细胞样乳腺癌组织中的表达量为2.07±0.95,显著高于配对癌旁组织的表达量(P<0.05)。结论基底细胞样乳腺癌组织的ADAM12L蛋白与核酸表达量均显著高于正常或良性乳腺组织,且与乳腺癌淋巴结转移和临床分期有着密切的联系。ADAM12L可能可以作为新的基底细胞样乳腺癌患者的个体化诊疗靶点。Objective To investigate the expression of ADAM12L protein and its nucleic acid in basal-like breast cancer and analyze its clinical significance.MethodsThe expression of ADAM12L protein in 86 cases of basal-like breast cancer and 20 cases of benign mammary fibroma were detected by immunohistochemistry.The real-time PCR method was used to detect the expression of ADAM12L m RNA in 60 cases of basal-like breast cancer tissue and adjacent tissue.ResultsImmunohistochemical staining showed that the expression of ADAM12L protein（84.9%,73/86）in basal-like breast cancer tissues was significantly higher than that in benign breast tissue samples（45%,9/20）,P0.05.The positive rate of ADAM12L in patients without lymph node metastasis was 47.8%,it’s lower than 73.8%the rate of the patients with positive lymph node metastasis（χ^2=5.073,P=0.024）.In clinical stageⅢ-Ⅳ,the positive rate of ADAM12L（74.5%,41/55）was higher than that of clinical stageⅠ-Ⅱ（35.5%,11/31）,χ^2=11.073,P0.01.With real-time PCR,the expression level of ADAM12L in basal-like breast cancer tissues was 2.07±0.95,which was significantly higher than that in adjacent tissues（P〈0.05）.Conclusion The expression of ADAM12L protein and nucleic acid in basal-like breast cancer tissues is significantly higher than that in normal or benign breast tissues,and is closely related to lymph node metastasis and clinical stage.ADAM12L may be used as a new target for individual diagnosis and treatment of the basal-like breast cancer patients.

关 键 词：基底细胞样乳腺癌 ADAM12L 预后 

分 类 号：R737.9[医药卫生—肿瘤] R361.2[医药卫生—临床医学]