桂花浸膏酶法制备工艺优化  

Optimization on preparation of Osmanthus extracts by enzymes treatment

作　　者：张雪松[1] 裴建军[2] 赵林果[2] 汤锋 房仙颖 ZHANG Xue-song;PEI Jian-jun;ZHAO Lin-guo;TANG Feng;FANG Xian-ying(College of Tea and Food Science and Technology,Jiangsu Polytechnic College of Agriculture and Forestry,Jurong,Jiangsu 212400,China;College of Chemical Engineering,Nanjing Forestry University,Nanjing,Jiangsu 210037,China;International（2entre Jot Bamboo and Rattan,Beijing 100714,China)

机构地区：[1]江苏农林职业技术学院,江苏句容212400 [2]南京林业大学,江苏南京210037 [3]国际竹藤中心,北京100714

出　　处：《食品与机械》2018年第6期180-186,共7页Food and Machinery

基　　金：国家林业局林业公益性行业科研基金专项(编号:201404601);江苏省生物质绿色燃料与化学品重点实验室开放基金(编号:JSBGFC14014)

摘　　要：为改进桂花浸膏制备工艺,提高桂花浸膏得率及品质,试验在多种风味水解酶中优选,确定采用β-葡萄糖苷酶和果胶酶复配对桂花进行酶处理,酶处理的桂花用石油醚提取制备桂花浸膏,产物采用GC-MS联用仪进行分析。利用响应面分析法优化复合酶法处理桂花制备桂花浸膏的工艺条件,并建立可靠的多元二次回归模型。结果表明,pH为4.7、酶解时间2.6h、酶解温度46℃、液料比19.8:1(mL/g)、复合酶添加量54.4IU/g、β-葡萄糖苷酶占总酶活的48.1%,该条件下桂花浸膏得率为3.32%,比直接提取所得浸膏得率提高了62.75%。同时GC-MS检测表明采用β-葡萄糖苷酶-果胶酶复配处理对桂花浸膏的品质有较大提升,浸膏中主要化合物的数量有所增加,与对照相比浸膏中二氢芳樟醇、γ-癸内酯、β-紫罗兰酮、二氢-β-紫罗兰酮、香叶醇、柠檬烯等主要香气物质的含量分别提高了27.27%,116.36%,100.00%,247.06%,72.84%,14.29%,并检测出橙花叔醇和β-紫罗兰醇。桂花经β-葡萄糖苷酶和果胶酶复配酶处理后,其浸膏得率及呈香品质均能有效提高。To hnprove the yield and quality of Osmanthus extracts, several techniques were applied including the enzymatic hydrolysis by β glucosidase and pectinase complex along with petroleum ether ex traction and GC-MS analysis. The enzymatic hydrolysis process was optimized usingresponse surface methodology, and a reliable multiple quadratic regression model was established. The results showed thatthe best levels of the factors were as followed： pH 4.7, time 2.6 h, temperature 46 ℃, ratio of material to liquid 19.8 ： 1 （mL/g）, and the amount of enzyme complex 54.4 IU/g with 48.1% β-glueosidase activity, which was confirmed by experiments wilh an extraction yield of 3.32% , an increment of 62.75% compared to direct extrac tion. The results of GC MS indicate an increase of main compounds, revealing that the hydrolysis by enzyme complex is more effective than direct extraction in terms of the extraction quality improvement. Compared with the control, the contents of the main aroma sub stances , such as dihydrolinalool, β-decalactone, dihydro-β ionone, β- ionone, geraniol, and limonene, were increased by 27. 27%, 116.36% , 100.00%, 247.06%, 72.84% and 14.29%, respectively. Meanwhile, nerolidol and β-ionol were also detected by GC MS. After treating by β- glucosidase-pectinase enzyme, the yield and aroma quality of Osmanthus abstracts improved effectively.

关 键 词：桂花 浸膏 复配酶 风味酶 

分 类 号：TQ654.2[化学工程—精细化工]