桑叶黄酮对糖尿病大鼠血糖水平的影响及机制探讨  

Effect and Mechanism of Flavonoids from Mori Folium on Blood Glucose Level in Diabetic Rats

作　　者：黄链莎[1] 刘铜华[2] 孙文[2] 许光远 郭璇[2] 李迎真[1] 陈淑惠[1] 周鹏[1] HUANG Lian-sha;LIU Tong-hua;SUN Wen;XU Guang-yuan;GUO Xuan;LI Ying-zhen;CHEN Shu-hui;ZHOU Peng(Baoan District Traditional Chinese Medicine Hospital,Shenzhen 518133,China;Key Laboratory of Health Cultivation of the Ministry of Education,Beijing University of Chinese Medicine,Beijing 100029,China;Fuxing Hospital,Capital Medical University,Beijing 100045,China)

机构地区：[1]深圳市宝安区中医院,广东深圳518133 [2]北京中医药大学教育部中医养生学重点实验室,北京100029 [3]首都医科大学附属复兴医院,北京100045

出　　处：《中国实验方剂学杂志》2018年第16期152-156,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省自然科学基金项目(2015A030310222)

摘　　要：目的：通过观察桑叶黄酮降血糖作用,探讨其作用机制。方法：8周龄雄性SD大鼠24只,链脲佐菌素（STZ）腹腔注射造模成功后,按血糖、体质量随机分为模型组、吡格列酮组、桑叶黄酮组,每组8只,另设同周龄正常SD大鼠8只为正常组。治疗6周后,检测大鼠的空腹血糖（fasting blood glucose,FBG）,总胆固醇（cholesterd,CHO）,甘油三酯（total triglyceride,TG）,游离脂肪酸（free fatty acids,FFA）,天门冬氨酸氨基转移酶（aspartate aminotransferase,AST）,血清胰岛素水平（fasting insulin,Fins）,计算胰岛素抵抗指数（homa insulin-resistance,HOMA-IR）;采用实时荧光定量PCR,蛋白免疫印迹法（Western blot）检测肝脏相关mRNA和蛋白表达。结果：与正常组比较,模型组大鼠FBG,Fins,HOMA-IR,体质量,CHO,TG,FFA明显升高（P〈0.05,P〈0.01）;肝脏蛋白激酶C（protein kinase C,PKC）mRNA和蛋白表达明显升高（P〈0.05,P〈0.01）,腺苷酸激酶2（adenylate kinase2,AK2）,过氧化物酶增殖物激活受体γ辅助激活因子1α（peroxisome proliferator activated receptor gamma coactivator 1-alpha,PGC1α）mRNA表达明显降低（P〈0.05,P〈0.01）。与模型组比较,桑叶黄酮组FBG,Fins,HOMA-IR,体质量,CHO,TG,FFA明显降低（P〈0.05,P〈0.01）;肝脏PKC mRNA和蛋白表达明显降低（P〈0.05,P〈0.01）,AK2,PGC-1αmRNA表达明显升高（P〈0.05,P〈0.01）。结论：桑叶黄酮具有降低血糖,FFA的作用,机制可能是通过抑制FFA诱导的PKC途径以及改善基于AK2,PGC1α表达的能量稳态发挥抗糖尿病效果。Objective： To elucidate the effect and mechanism of flavonoids from Mori Folium（FM） on improving blood glucose in diabetic rats. Method： After successful modeling by intraperitoneal injection of streptozotocin（STZ）,twenty-four 8-week-old male SD rats were randomly divided into model group,Pioglitazone group,FM group with 8 rats in each group according to their levels of blood glucose and body weight,and another8 normal SD rats with the same age were also used as a normal group. After treatment for 6 weeks,their fasting blood glucose（FBG）, cholesterd（CHO）, total triglyceride（TG）, free fatty acids（FFA）, aspartate aminotransferase（AST）,fasting insulin（Fins）,and homa insulin-resistance（HOMA-IR） were measured. Realtime fluorescence quantitative polymerase chain reaction（Real-time PCR） and Western blot were used to quantify the mRNA and protein expressions of certain targets in the liver. Result： As compared with the normal group,the body weight,FBG,Fins,HOMA-IR,CHO,TG,FFA were significantly increased in model group（P〈0. 05,P〈0. 01）; the mRNA and protein expressions of protein kinase C（PKC） were increased significantly in model group（P〈0. 05, P〈0. 01）, but the mRNA expressions of adenylate kinase2（AK2）, and peroxisome proliferator activated receptor gamma coactivator 1-alpha（PGC-1α） were decreased significantly（P〈0. 05,P〈0. 01）. As compared with the model group,body weight,FBG,Fins,HOMA-IR,CHO,TG,and FFA were significantly decreased in FM group（P〈0. 05,P〈0. 01）; the mRNA and protein expressions of PKC in liver tissues were decreased significantly（P〈0. 05,P〈0. 01）,but the mRNA expressions of AK2,and PGC-1α were increased significantly in FM group（P〈0. 05,P〈0. 01）. Conclusion： It was demonstrated that FM may play an anti-diabetic effect by inhibiting the PKC pathway induced by FFA and improving the energy homeostasis based on the expression of AK2 and PGC-1α.

关 键 词：桑叶黄酮 2型糖尿病 游离脂肪酸 蛋白激酶C 腺苷酸激酶2 过氧化物酶增殖物激活受体γ辅助激活因子1α 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学]