液泡分选蛋白4B在小鼠牙胚发育的时空表达  

The spatio-temporal expression of VPS4B during molar tooth germ development in mice

作　　者：陈栋 李晓聪 鲁方丽 王莹莹[1] 李强[1] CHEN Dong;LI Xiaocong;LU Fangli;WANG Yingying;LI Qiang(450052,Department of Stomatology,the First Affiliated Hospital of Zhengzhou University,Chin)

机构地区：[1]郑州大学第一附属医院口腔科,450052

出　　处：《实用口腔医学杂志》2018年第4期442-445,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81470033)

摘　　要：目的:研究液泡分选蛋白4B(vacuolar protein sorting 4B,VPS4B)在小鼠磨牙发育不同时期的表达。方法:取胚胎13.5、14.5、16.5 d的胎鼠头部及出生后2.5、7 d的幼鼠下颌骨组织,石蜡包埋后获取第一磨牙牙胚组织切片,通过组织化学染色确定各组牙胚的发育时期,免疫组织化学法检测牙胚中VPS4B的表达分布。结果:胚胎13.5、14.5、16.5 d小鼠第一磨牙牙胚分别处于蕾状期、帽状期、钟状早期,出生后2.5、7 d分别处于分泌期和矿化期;VPS4B表达于小鼠磨牙发育的整个过程,并在构成牙胚的多种细胞中有不同程度的表达。随发育的进行,成釉细胞和成牙本质细胞中VPS4B呈强阳性持续表达,而牙囊细胞表达逐渐减弱。结论:VPS4B表达于正常小鼠牙胚发育过程中且随发育进行表达发生改变,VPS4B可能参与牙胚的正常发育。Objective： To verify the expression change of vacuolar protein sorting 4 B（ VPS4 B） in different developmental stages of mouse molar tooth germ. Methods： The paraffin tissue sections of the first molar tooth germ were obtained from the head of the fetal mouse at the embryonic stage of 13. 5,14. 5 and 16. 5 days,and the mandible of the larvae aged 2. 5 and 7 days after birth. The histochemical staining was used to identify tooth germ development period while the expression and location of VPS4 B was detected by immunohistochemical method. Results： The first molars tooth germs of the embryos at 13. 5,14. 5 and 16. 5 days were in the bud,cap and the early bell-shaped stage respectively,while those of the 2. 5 days of age was in the secretory stage and the 7 days in the mineralization period. VPS4 B was expressed in the whole development progress of mouse molar germ,and different levels of expression were found in various types of cells forming the mouse molar tooth germ. VPS4 B was strongly expressed in ameloblasts and odontoblasts,but as the development progressed,the expression in the dental follicle cells was gradually reduced. Conclusion： VPS4 B expresses at different stages of tooth germ in normal mice,so VPS4 B may be involved in the normal development of tooth germ.

关 键 词：小鼠牙胚 发育 免疫组化 液泡分选蛋白4B(VPS4B) 

分 类 号：Q344[生物学—遗传学] R78[医药卫生—口腔医学]