季铵盐包裹溴化银纳米复合物改性树脂基质的抗菌及机械性能评价  

The antibacterial activity and mechanical properties of AgBr/BHPVP nanocomposite modified resin matrix

作　　者：陈银燕 张瑜[1] 柴治国 沈丽娟 邢晓东[3] 肖玉鸿[1] CHEN Yinyan;ZHANG Yu;CHAI Zhiguo;SHEN Lijuan;XING Xiaodong;XIAO Yuhong(650032,Department of Stomatology,Kunming General Hospital of Chengdu Military Command,Teaching Hospital of Kunming Medical University,China;State Key Laboratory of Military Stomatology,Department of Prosthodonties,School of Stomatology,The Fourth Military Medical University,Xi＇ an;College of Chemical Engineering,Nanjing Univer-sity of Science and Technology)

机构地区：[1]昆明医科大学教学医院成都军区昆明总医院口腔科,650032 [2]军事口腔医学国家重点实验室陕西省口腔医学重点实验室第四军医大学口腔医院修复科 [3]南京理工大学化工学院

出　　处：《实用口腔医学杂志》2018年第4期451-455,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号：81460107)

摘　　要：目的:探讨季铵盐包裹溴化银纳米复合物(Ag Br/BHPVP)改性树脂基质的抗菌及机械性能。方法:将变形链球菌分别培养在含有0(对照组)、0.5%、1.0%和1.5%Ag Br/BHPVP的树脂试件表面,菌落形成单位(CFU)计数法检测试件表面变形链球菌存活数,激光共聚焦显微镜(CLSM)观察试件表面细菌生物膜活/死菌染色情况,再分别检测试件挠曲强度、维氏显微硬度。结果:老化处理前后,1.0%Ag Br/BHPVP组CFU均显著低于对照组(P<0.05),且自身老化前后CFU之间无显著差异(P>0.05),CLSM观察可见改性组试件表面黏附的活菌量少于对照组,1.0%组试件挠曲强度及维氏显微硬度值与对照组间均无显著性差异(P>0.05)。结论:经1.0%Ag Br/BHPVP改性的树脂基质对变形链球菌具有稳定、长效抗菌活性,且机械性能无显著影响。Objective： To study the antibacterial activity and mechanical properties of Ag Br/BHPVP nanocomposite modified resin matrix. Methods： Streptococcus mutans（ S. mutans） UA 159 were cultured on the resin specimens containing 0（ the control）,0. 5%,1. 0%,1. 5% of Ag Br/BHPVP respectively. The colony-forming units（ CFU） of S. mutans before and after 3 month aging were quantified,and their survival status was observed by confocal laser scanning microscope（ CLSM）. Flexural strength and Vickers microhardness were measured respectively. Results： The CFU of S. mutans on resin specimens of 1. 0% Ag Br/BHPVP groups was significantly lower than that of control groups before and after 3 month aging（ P 〈 0. 05）. The CFU on resin specimens of 1. 0% Ag Br/BHPVP group had no significant difference between before and after 3 month aging（ P 〉 0. 05）. CLSM observation revealed that alive bacteria on resin specimens of modified groups were significantly less than those of control groups. There was no significant difference in the value of flexural strength and microhardness between 1. 0% Ag Br/BHPVP and control groups（ P 〉 0. 05）. Conclusion： 1. 0% Ag Br/BHPVP modified resin matrix has long-lasting antibacterial activity against S. mutans and inherent mechanical properties.

关 键 词：季铵盐 溴化银 树脂基质 抗菌活性 机械性能 

分 类 号：R783.1[医药卫生—口腔医学]