eNOS基因治疗促进大鼠缺血后肢的血管新生  

eNOS Gene Therapy Enhance Neovascularization in Rat Ischemic Hindlimb

作　　者：宋礼坡[1] 郭连瑞[1] 张建[1] 谷涌泉[1] 王春梅[1] 吴英锋[1] 罗涛[1] 崔世军[1] 李建新[1] 汪忠镐[1] SONG Li-po;GUO Lian-rui;ZHANG Jian;GU Yong-quan;WANG Chun-mei;WU Ying-feng;LUO Tao;CUI Shi-jun;LI Jian-xin;WANG Zhong-gao(Department of Vascular Surgery,Xuan Wu Hospital,and Institute of Vascular Surgery,Capital Medical University,Beijing,100053,China)

机构地区：[1]首都医科大学宣武医院血管外科首都医科大学血管外科研究所,北京100053

出　　处：《现代生物医学进展》2018年第11期2001-2005,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(30471708)

摘　　要：目的:评估内皮型一氧化氮合酶(Endothelial nitric oxide synthase,eNOS)基因治疗对大鼠缺血后肢血管新生的影响。方法:局麻下将30只雄性SD大鼠后肢缺血模型制作后,随机分成实验组和对照组,每组15只。模型制作后1周,采用肌肉注射的方法,实验组缺血后肢接受载有eNOS基因的5型重组腺病毒治疗,对照组接受生理盐水治疗。eNOS基因治疗后4周,评估SD大鼠踝部动脉压、微循环灌注、数字减影血管造影、组织微血管计数以及eNOS蛋白的表达。结果:eNOS基因治疗后4周,和对照组相比,接受eNOS基因治疗的大鼠缺血肢体,表现了更好的血流恢复(踝部动脉压(mmHg):58.2±4.7 vs 86.8±4.3,P<0.01;微循环灌注:142.0%±21.5%vs 219.6%±26.2%,P<0.01)、侧枝开放和血管新生(血管造影:6.7±1.1 vs 14.4±1.7,P<0.01;微血管/肌纤维比值:0.34±0.03 vs 0.56±0.02,P<0.01)以及eNOS蛋白的高表达(0.46±0.02 vs 0.73±0.02,P<0.01)。结论:eNOS基因治疗促进SD大鼠缺血后肢的血管新生。Objective： This study was to evaluate the effect of eNOS gene therapy on neovascularization in ischemic hindlimb of SD rat. Methods： Thirty ischemic hindlimb models of male SD rats were made under anesthesia. They were randomly divided into experiment and control group. Each group had 15 rats. One week later, experiment group received eNOS gene therapy and control group received normal saline therapy. Neovascularization in ischemic hindlimb was evaluated 4 weeks post gene therapy. Assessment of ischemic hindlimbs included ankle artery pressure, microcirculation perfusion, digital subtraction arteriography, histological capillary count, and eNOS protein expression. Results： Compared with control group, ischemic hindlimbs which received eNOS gene tharapy showed better blood recovery（ankle artery pressure（mm Hg）： 58.2±4.7 vs 86.8±4.3, P〈0.01; tissue microcirculation perfusion： 142.0%±21.5% vs 219.6%±26.2%, P〈0.01）, lateral branch opening and angiogenesis（digital subtraction arteriography： 6.7±1.1 vs 14.4±1.7,P〈0.01; capillary/muscle fibers ratio： 0.34±0.03 vs 0.56±0.02, P〈0.01）, and higher eNOS expression（0.46±0.02 vs 0.73±0.02, P〈0.01）. Conclusions： eNOS gene therapy can enhance neovascularization in ischemic hindlimb of SD rat.

关 键 词：内皮型一氧化氮合酶 基因治疗 严重肢体缺血 血管新生 

分 类 号：R-33[医药卫生] Q813[生物学—生物工程]