干扰素IFNL4对巨噬细胞系THP-1免疫应答的影响  

The Effects of IFNL4 Expression on Immune Response of Macrophages in THP-1 Cell System

作　　者：党引利 徐溪 陈丽展[1] 张瑶[1] 白丹 吴朔[1] DANG Yin-li;XU Xi;CHEN Li-zhan;ZHANG Yao;BAI Dan;WU Shuo(Respiration Department,Xi Jing hospital,Fourth Military Medical University,Xi＇an,Shaanxi,710032,China;Xi＇an Jiaotong University,Department of Biochemistry and Molecular Biology,Xi＇an,Shaanxi,710056,China)

机构地区：[1]第四军医大学西京医院呼吸科,陕西西安710032 [2]西安交通大学生物化学与分子生物学系,陕西西安710056

出　　处：《现代生物医学进展》2018年第11期2047-2051,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81601553)

摘　　要：目的:分析Interferon-lambda 4(IFNL4)表达与巨噬细胞免疫应答间的关系,探讨IFNL4调控免疫应答的信号机制,发掘IFNL4在免疫调理方面的潜在应用价值。方法:建立THP-1细胞培养及分化刺激体系,使用RT-PCR检测不同分化状态THP-1细胞IFNL4的表达水平,并在THP-1细胞中过表达IFNL4,检测IFNL4过表达对THP-1细胞分泌IL-12、TNF-α、IL-10和TGF-β等细胞因子及细胞迁移效率的影响。结果:THP-1细胞分化抑制IFNL4的表达,分化前比分化后IFNL4表达水平相对定量下降255.46倍,差异显著性P<0.001;M2极化巨噬细胞较M1细胞表达IFNL4因子水平升高14.69倍,显著性差异P=0.009;IFNL4过表达可抑制IL-12和TNF-α表达水平,其中TNF-α表达水平变化具有统计学意义(P=0.017),表达水下降5.97倍。IFNL4可促进IL-10和TGF-β的表达,其中TGF-β变化具有统计学意义(P=0.046),表达水平相对上升2.42倍。且IFNL4对THP-1细胞迁移效率具有抑制作用(P=0.005),刺激前细胞迁移数为45.33,IFNL4刺激后迁移数为32.67,迁移移效率下降1.39倍。结论:干扰素IFNL4在分化的M2型THP-1巨噬细胞中具有较高的表达水平,且对THP-1的免疫应答具有一定的抑制作用。Objective： To investigate the relationship between IFNL4 expression and macrophage functions, analyze the role of IFNL4 in immune regulation, and explore for the potential application value of IFNL4 in immune regulation. Methods： The THP-1 cell culture and stimulation system was esteblished. The IFNL4 expressions in different type of THP-1 cells were detected by RT-PCR.IFNL4 was then overexpressed in THP-1 cells, and the expression of IL-12, TNF-α, IL-10 and TGF-β in these cells were analyzed by RT-PCR. The migration efficiency of THP-1 cells was analyzed by transwell assay before or after IFNL4 overexpression. Results： The differentiation of THP-1 cells inhibited IFNL4 expression（P〈0.001）, and M2 macrophage can secrete more IFNL4 comparing to M1 macrophage（P=0.009）. IFNL4 overexpression in THP-1 cells inhibit IL-12 and TNF-α secretion while promote IL-10 and TGF-βexpression, in which TNF-α and TGF-β variations were significantly different（P=0.017 and p=0.046, respectively）. Furthermore, IFNL4 overexpression inhibit THP-1 cell migration significantly（P=0.005）. Conclusion： IFNL4 has a certain inhibitory effect on inflammation function of macrophage in THP-1 cell system.

关 键 词：IFNL4 THP-1巨噬细胞 免疫应答 细胞因子 巨噬细胞迁移 

分 类 号：R-33[医药卫生] R392.12