大围山微型鸡和艾维茵肉鸡PFKM基因表达量与pH值及肌糖原含量相关性研究  

Correlation Analysis on PFKM Gene Expression,pH Value,Muscle Glycogen Content in Daweishan Mini Chicken and Avian Broiler

作　　者：王坤[1] 豆腾飞 徐志强[1,2] 荣华[1] 刘丽仙[1] 黄英[1] 李琦华[1] 葛长荣[1,2] 贾俊静[1] WANG Kun;DOU Tengfei;XU Zhiqiang;RONG Hua;LIU Lixian;HUANG Ying;LI Qihua;GE Changrong;JIA Junjing(College of Animal Science and Technology,Yunnan Yunnan Agricultural University,Kunming,Yunnan 650201;College of Food Science and Technology,Agricultural University,Kunming,Yunnan 650201)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南农业大学食品科学技术学院,云南昆明650201

出　　处：《中国家禽》2018年第14期10-13,共4页China Poultry

基　　金：国家自然科学基金项目(U1702232;31560618;31572359);云南省自然科学基金重点项目(2016FA046);"十三五"农村领域国家科技计划课题(2015BAD03B03-4);云南省科技厅南希莱恩院士工作站(2017IC048);云岭产业技术领军人才项目;云南农业大学学生科技创新创业行动基金项目(2018ZKX036)

摘　　要：为研究家禽肌肉磷酸果糖激酶(PFKM)m RNA表达量与p H_(45min)值及肌糖原含量的相关性,检测了大围山微型鸡和艾维茵肉鸡不同日龄肌肉组织中PFKM mRNA表达量、pH_(45min)值和肌糖原含量。结果显示:大围山微型鸡肌肉中PFKM mRNA表达量与肌糖原含量整体上均高于艾维茵肉鸡,而pH_(45min)值则是艾维茵肉鸡高于大围山微型鸡;PFKM mRNA表达量与pH_(45min)值呈显著负相关(P<0.05),与肌糖原含量呈正相关;肌糖原含量与pH_(45min)值呈负相关,但相关性不显著(P>0.05)。研究表明PFKM基因可能是影响家禽肉品质的重要候选基因。In order to study the correlations between phosphofructokinase（PFKM） m RNA expression,p H（45min）value and muscle glycogen content in poultry, Daweishan Mini chicken and Avian broiler were selected to detect PFKM m RNA expression, p H（45min）value and muscle glycogen content in muscle tissue. The results showed that PFKM m RNA expression and glycogen content in Daweishan Mini chicken were higher than those in Avian broiler, but p H（45min）value of Avian broiler was higher than that of Daweishan Mini chicken. PFKM m RNA expression was significantly negative correlated with p H（45min）value（P〈0.05） and positively correlated with muscle glycogen content, but there was negative correlation between muscle glycogen content and p H（45min）value（P〉0.05）. It was concluded that PFKM gene might play an important role in regulating glycogen content in chickens, and could be selected as a candidate gene of meat quality.

关 键 词：磷酸果糖激酶 大围山微型鸡 艾维茵肉鸡 肌糖原含量 PH值 

分 类 号：S831-2[农业科学—畜牧学]