5-羟色胺在粘虫视叶中的分布  

Distribution of 5-hydroxytryptamine in the optic lobes of the oriental armyworm,Mythimna separata( Lepidotpera: Noctuidae)

作　　者：郭培[1] 马百伟 赵新成[1] 王高平[1] 谢桂英[1] GUO Pei;MA Bai-Wei;ZHAO Xin-Cheng;WANG Gao-Ping;XIE Gui-Ying(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,Chin)

机构地区：[1]河南农业大学植物保护学院,郑州450002

出　　处：《昆虫学报》2018年第6期668-675,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(U1604109);河南省豫南植物有害生物绿色防控院士工作站项目(YZ201504)

摘　　要：【目的】解剖分析粘虫Mythimna separata成虫视叶的结构,研究5-羟色胺(5-hydroxytryptamine,5-HT)在视叶中的分布。【方法】采用组织包埋切片技术和免疫组织化学方法,使用突触蛋白抗体标记粘虫成虫视叶内神经髓结构,用抗5-HT血清标记5-HT;利用激光共聚焦扫描显微镜照相获取数码图像,利用图形分析软件识别神经髓结构及进行细胞体群分组与计数。【结果】粘虫成虫的视叶由视神经节层、视髓、副视髓、视小叶和视小叶板5个神经髓结构组成。免疫染色显示粘虫视叶内具有5-HT,每个视叶内约具有40个5-HT免疫标记的细胞体,这些细胞体分为3个细胞体群,位于视髓前方或腹面的内侧。视叶内所有的神经髓结构均含有5-HT免疫标记神经纤维。视神经节层的5-HT免疫标记神经纤维来自视叶的切向神经元,视髓的5-HT免疫标记神经纤维主要来自视叶的切向神经元、远心神经元和无长突神经元。视髓呈明显分层现象,主要分为3层,其中中间一层5-HT免疫标记神经纤维较密集。副视髓存在少量的5-HT免疫标记神经纤维。视小叶和视小叶板中的5-HT免疫标记神经纤维来自远心神经元,分为明显的2层,其中有少量神经纤维投射至视髓,将视小叶、视小叶板和视髓连接起来。【结论】粘虫视叶中广泛分布着5-HT免疫标记神经纤维。该研究结果为进一步研究5-HT在视觉机制中的作用奠定一定的解剖学基础。【Aim】This study aims to investigate the anatomy of the optic lobes of adults of the oriental armyworm,Mythimna separata（ Lepidotpera： Noctuidae） and the distribution of 5-hydroxytryptamine（ 5-HT） in the neuroplis of the optic lobes. 【Methods 】 The tissue embedding and section,immunohistochemical staining with synaptic protein antibody and anti-5-HT serum were used to label the neuropil structure of the optic lobe and 5-HT in M. separata adults,respectively. The digital images of the optic lobes were obtained by using laser scanning confocal microscopy. The identification of neuropils and the grouping and counting of 5-HT immunoreactive（ 5-HTi） cell bodies were performed using image software. 【Results】The optic lobe of M. separata adults is composed of five neuropils,i. e.,lamina,medulla,accessory medulla,lobula,and lobula plate. There are about 40 5-HTi cell bodies in each optic lobe,and all the five neuropil regions contain 5-HTi neural processes. 5-HTi neural processes in lamina are originated from the tangential neurons of the optic lobe,and the processes of medulla are fromthe tangential neurons,centrifugal neurons and amacrine neurons. Medulla is distributed into mainly three layers,in which the middle layer possesses the densest 5-HTi processes. A few of 5-HTi processes exist in accessory medulla. In the lobula and lobula plate,the 5-HTi processes are originated from the centrifugal neurons. At least two layers of 5-HTi processes in lobula and lobula plate were observed. A few of neural processes project to medulla and connect lobula,lobula plate and medula. 【Conclusion】5-HT immunoreactive fibers are widely distributed in the optic lobe of M. separata. The results provide the basic knowledge of neural anatomy for further studying the role of 5-HT in the visual system of M.separata.

关 键 词：粘虫 视叶 神经元 神经纤维 5-羟色胺 免疫染色标记 

分 类 号：Q964[生物学—昆虫学]