基于Luminex液相芯片检测原发性开角型青光眼房水中多种细胞因子  

Determination of multiple cytokines in aqueous humor of primary open angle glaucoma based on Luminex multiplex immunoassay

作　　者：张勇 杨钦媚 郭凤 陈侠 谢琳 ZHANG Yong;YANG Qin-mei;GUO Feng;CHEN Xia;XIE Lin(Department of Ophthalmology, Institute of Surgery Re-search, Daping Hospital, Army Medical Universit;Chongqing Key Laboratory of Ophthalmology, Chongqing 400042, China)

机构地区：[1]解放军陆军军医大学第三附属医院(野战外科研究所)眼科,重庆400042 [2]重庆市眼科科学重点实验室,重庆400042 [3]重庆医科大学附属第三医院眼科,重庆400042

出　　处：《眼科》2018年第4期281-285,共5页Ophthalmology in China

摘　　要：目的应用Luminex液相芯片检测原发性开角型青光眼(POAG)患者房水中多种细胞因子浓度,并分析这些因子的改变与疾病的关系。设计实验研究。研究对象24例POAG患者及同年龄段22例老年性白内障患者的房水样本。方法使用Luminex液相芯片检测房水中10种细胞因子浓度,比较POAG和白内障患者房水中细胞因子浓度的差异。主要指标血小板衍生生长因子-AA(PDGF-AA)、血小板衍生生长因子-AB(PDGF-AB)、表皮生长因子(EGF)、血管内皮生长因子(VEGF)、中性粒细胞集落刺激因子(G-CSF)、白介素-6(IL-6)、白介素-8(IL-8),趋化因子配体5(RANTES)、干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)的浓度。结果 POAG患者房水中PDGF-AB(P=0.040)、EGF(P=0.048)、VEGF(P=0.037)、G-CSF(P=0.029)和IL-6(P=0.018)的水平显著低于老年性白内障患者;PDGF-AA浓度显著高于白内障患者(P=0.001);IL-8、RANTEs、IFN-γ和TNF-α浓度两组间无显著差异(P均>0.05)。POAG患者房水中IL-6(P<0.001,ρ=-0.778)、IL-8(P=0.001,ρ=-0.623)和TNF-α(P<0.001,ρ=-0.685)的浓度与视野平均缺损值(MD)显著相关。结论 POAG患者房水中多种细胞因子浓度低于同年龄段老年性白内障患者,其能否反映视神经和小梁网的损害情况有待进一步研究。Objective To measure cytokine concentrations in aqueous humor of patients with primary open-angle glaucoma （POAG） and cataract with the Luminex multiplex immunoassay. Analyze the relationship between these cytokines and disease. Design Experimental study. Participants This study included aqueous humor of 24 eyes with POAG and 22 eyes with cataract. Methods The concentrations of nine cytokines in aqueous humor were measured using the Luminex suspension array technique. The clinical charac- teristics of patients were also obtained for correlation analysis. Main Outcome Measures The concentrations of platelet derived growth faetor-AA （PDGF-AA）, platelet derived growth factor-AB （PDGF-AB）, epidermal growth factor（EGF）, vascular endothelial growth factor （VEGF）, granulocyte colony-stimulating factor （G-CSF）, interleukin-6 （IL-6）, interleukin-6 （IL-8）, regulated on activation, normal T cell expressed and secreted （RANTES）, interferon-γ（IFN-γ） and tumor necrosis factor-α （TNF-α in the aqueous humor. Results Compared with the cataract group, the levels of PDGF-AB （P=-O.040）, EGF （P=0.048）, VEGF （P=0.037）, G-CSF （P=0.029） and IL-6 （P=O.018） were significantly lower in the aqueous humor of POAG. The level of PDGF-AA was significantly higher in POAG patients than in cataract patients. The level of IL-8, RANTEs, IFN-γ and TNF-α did not differ among the groups （all P〉0.05）. Mean deviation （MD） values were significantly associated with the levels of IL-6 （P〈0.001, p=-0.778）, IL-8 （P=0.001, p=-0.623） and TNF-α （P〈0.001, p=-0.685）. Con- clusion Cytokine levels in the aqueous humor of POAG patients are lower than in cataract patients, and whether this reflects the dam-age of optic nerve and trabecular meshowrk is worthy of further study. （Ophthalmol CHN, 2018, 27： 281-285）

关 键 词：青光眼 房水 细胞因子 

分 类 号：R775.2[医药卫生—眼科]