RNA干扰敲低β-catenin的表达对瘢痕疙瘩成纤维细胞增殖及凋亡的影响  

Effects of β-catenin on the Proliferation and Apoptosis of Keloid Fibroblast Cells

作　　者：荣媛[1] 刘明华[1] 邓朝霞[1] 任小宝[1] 向强[1] RONG Yuan;LIU Ming-hua;DENG Zhao-xia;REN Xiao-bao;XIANG Qiang(Department of emergency,Southwest Hospital in Third Military Medical University,Chongqing,400038,Chin)

机构地区：[1]解放军第三军医大学西南医院急诊科,重庆400038

出　　处：《现代生物医学进展》2018年第14期2631-2636,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81071537)

摘　　要：目的:探讨β-catenin对人瘢痕疙瘩成纤维细胞(keloid fibroblasts,KFB)增殖和凋亡的影响。方法:分别采用Real-time PCR(RT-PCR)和Western blot检测正常组织和瘢痕疙瘩中β-catenin的mRNA及蛋白表达。将针对人β-catenin基因设计合成的3对特异性小干扰RNA(siRNA)分别转染体外培养的人瘢痕疙瘩成纤维细胞,通过RT-PCR和Western blot筛选出干扰人瘢痕成纤维细胞β-catenin基因表达的最佳siRNA。通过siRNA沉默β-catenin表达后,采用MTT法检测KFB的增殖情况,流式细胞仪检测细胞凋亡率。结果:瘢痕疙瘩成纤维细胞中β-catenin的表达较正常组织明显升高(P<0.05)。通过转染β-catenin siRNA降低其表达后,成纤维细胞的增殖能力明显下降(P<0.05),凋亡水平显著增高(P<0.05)。结论:沉默β-catenin能够显著抑制瘢痕疙瘩成纤维细胞增殖并促进其凋亡。Objective： To investigate the effect of β-catenin siRNA on the proliferation and apoptosis of keloid fibroblast cell.Methods： Real-time PCR and Western blot were performed to monitor the mRNA and protein expression of β-catenin in pathological scar tissue and adjacent normal tissue. Keloid fibroblast cell in vitro were transfected with 3 pairs of specific β-catenin small interfering RNA（siRNA）, then Real-time PCR and Western blot were performed to identify the best siRNA. The proliferation and apoptosis of KFB transfected with β-catenin siRNA were detected by using MTT assay and flow cytometry. Results： The RT-PCR and Western blot assay results showed that the expression of β-catenin mRNA and protein in pathological scar tissue were significantly higher than those in the adjacent normal tissue（P〈0.05）. The β-catenin siRNA 2 remarkably inhibited the expression of β-catenin at mRNA and proteins in the Human keloid fibroblasts. Compared with the control group, cell proliferation was decreased and apoptotic rate were increased inβ-catenin siRNA 2 group. Conclusion： Knockdown of β-catenin significantly decreased the proliferation and increased the apoptosis of KFB, which could inhibit the formation of pathological scar.

关 键 词：瘢痕疙瘩 Β-CATENIN 增殖 凋亡 

分 类 号：R-33[医药卫生] R62