髓系细胞触发受体-1在脂多糖急性肺损伤大鼠肺组织中的表达及其与内质网应激和炎性反应的关系  

Expression of Triggering Receptor Expressed on Myeloid Cells-1 in Lung Tissue of Rats with Acute Lung Injury Induced by Lipopolysaccharide and Its Relationship with Endoplasmic Reticulum Stress and Inflammatory Response

作　　者：蒋虹[1] 李隽[1] 李斌 操明志[1] 童皖宁[1] JIANG Hong;LI Jun;LI Bin;CAO Ming-zhi;TONG Wan-ning(Department of Thoracic Surgery,411st Hospital of PLA,Shanghai,200081,China)

机构地区：[1]解放军第411医院胸外科,上海200081

出　　处：《现代生物医学进展》2018年第14期2657-2660,2677,共5页Progress in Modern Biomedicine

摘　　要：目的:研究髓系细胞触发受体-1(TREM-1)在脂多糖急性肺损伤(ALI)大鼠肺组织中的表达及其与内质网应激和炎性反应的关系。方法:选择成年雄性SD大鼠100只作为研究对象,将其分成ALI组(n=60)、对照1组(n=15)、观察组(n=20)和对照2组(n=5)。对比ALI组和对照1组TREM-1、内质网应激表达及肿瘤坏死因子-α(TNF-α)水平、Smith评分,对比观察组和对照2组的CHOP mRNA、GRP mRNA表达情况,并分析TREM-1与内质网应激、炎性反应和Smith评分的相关性。结果:ALI组6h、12h、1d及2d时间点的TREM-1 mRNA、CHOP mRNA、GRP78 mRNA、TREM-1、TNF-α水平及Smith评分均高于对照1组,且ALI组随着时间的推移,TREM-1 mRNA、CHOP mRNA、GRP78 mRNA、TREM-1、TNF-α水平及Smith评分均呈升高的趋势,在1d时达到最高,然后呈下降趋势(P<0.05)。观察组6h、12h、1d及2d时间点的CHOP mRNA、GRP mRNA表达高于对照2组,且观察组随着时间的推移CHOP mRNA、GRP mRNA表达均呈升高的趋势,在1d时达到最高,然后呈下降趋势(P<0.05)。根据Spearman法分析相关性发现,TREM-1 mRNA及TREM-1水平均与CHOP mRNA、GRP78 mRNA及Smith评分呈正相关,且TREM-1水平与TNF-α呈正相关(P<0.05)。结论:TREM-1在脂多糖ALT大鼠肺组织中的表达较高,且参与ALT肺部炎性反应,激活TREM-1可增强巨噬细胞内质网应激。Objective： To investigate the expression of triggering receptor expressed on myeloid cells-1（TREM-1） in lung tissue of rats with acute lung injury（ALI） induced by lipopolysaccharide and its relationship with endoplasmic reticulum stress and inflammatory response. Methods： 100 adult male SD rats were selected as research subjects, and they were divided into ALI group（n=60）, control 1 groups（n=15）, observation group（n=20） and control 2 group（n=5）. The expression of TREM-1 and endoplasmic reticulum stress, the level of tumor necrosis factor-α（TNF-α） and the score of Smith were compared between the ALI group and control 1 group. The expression of CHOP mRNA and GRP mRNA in the observation group and the control 2 groups were compared. The correlation between TREM-1 and endoplasmic reticulum stress, inflammatory response and Smith score was analyzed. Results： The levels of TREM-1 mRNA,CHOP mRNA, GRP78 mRNA, TREM-1,TNF-α and Smith scores in the ALI group at 6 h, 12 h, 1 d, 2 d were significantly higher than those in the control 1 group（P〈0.05）, and TREM-1 mRNA, CHOP mRNA, GRP78 mRNA,TREM-1,TNF-α and Smith scores in the ALI group were increased with the time, which were reached the highest level at 1 d, then there had a downward trend（P〈0.05）. The expression of CHOP mRNA and GRPmRNA in the observation group at 6 h, 12 h, 1 d and 2 d was higher than that of the control 2 group, the expression of CHOP mRNA and GRP mRNA in the observation group increased with the time, which were reached the highest level at 1 d, then there had a downward trend（P〈0.05）. According to the correlation analysis of Spearman method showed that TREM-1 mRNA and TREM-1 levels were positively correlated with CHOP mRNA, GRP78 mRNA and Smith score, and TREM-1 level was positively correlated with TNF-α（P〈0.05）. Conclusion： The expression of TREM-1 is higher in the lung tissue of rats with ALI induced by lipopolysaccharide, and it is involved in the inflammatory response of the ALT lung. Act

关 键 词：髓系细胞触发受体-1 脂多糖 急性 肺损伤 大鼠 表达 内质网应激 炎性反应 关系 

分 类 号：R-33[医药卫生] R655.3