MiRNA-155通过调控β-catenin促进结肠癌SW480细胞的侵袭  

MicroRNA-155 Promotes the Invasion of Colon Cancer Cell SW480 by Upregulating Wnt/β-catenin Signaling Pathway

作　　者：马志勇 李雪平 侯振宇 汪明强 吕金利 MA Zhi-yong;LI Xue-ping;HOU Zhen-yu;WANG Ming-qiang;LV Jin-li(No.153 Central Hospital of PLA,Zhengzhou,Henan,450042,China)

机构地区：[1]解放军第一五三中心医院普外科,河南郑州450042

出　　处：《现代生物医学进展》2018年第12期2242-2247,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30972894)

摘　　要：目的:探讨micro RNA-155(miR-155)对结肠癌细胞SW480侵袭能力的影响及其可能机制。方法:采用反转录-聚合酶链反应(RT-PCR)测定结肠癌组织与邻近正常结肠组织中miR-155的表达。将miR-155 mimic和β-catenin特异性的siRNA(β-catenin si RNA)分别通过脂质体转染法转染入结肠癌SW480细胞,应用RT-PCR检测细胞中miR-155和β-catenin m RNA的表达,采用蛋白质印迹法(Western Blot)检测β-catenin蛋白表达,采用Transwell侵袭实验检测miR-155 mimic及β-catenin si RNA对SW480细胞侵袭能力的影响。结果:结肠癌组织中的miR-155的表达较邻近正常结肠组织明显升高(P<0.05);miR-155 mimic可使β-catenin的m RNA和蛋白表达均显著升高(P<0.05),同时可显著增强SW480细胞的侵袭能力(P<0.05),而转染miR-155 mimic和β-catenin si RNA的SW480细胞侵袭能力较仅转染miR-155 mimic的SW480细胞显著减弱(P<0.05)。结论:结肠癌组织中miR-155的表达上调,可能通过激活B-catenin信号通路促进肿瘤细胞的远处侵袭转移。Objective： To investigate the role of micro RNA-155（miR-155） in the invasion potential of colon cancer cell and the exact underlying mechanism. Methods： The expression level of miR-155 in colon cancer and adjacent normal tissues was detected by realtime quantitative PCR（RT-PCR）. miR-155 mimics（miR-155）, or si RNA against β-catenin（β-catenin si RNA） were transfected into human colon cancer cell line SW-480 respectively using lipofectamine 2000, and RT-PCR was used to measure the m RNA expression levels of miR-155 and β-catenin, and β-catenin protein expression level was detected by Western blot. The in-vitro cell invasion ability was determined by Transwell invasion assays after up-regulating miR-155 or knocking down of β-catenin. Results： The expression levels of miR-155 was higher in colon cancer tissue compared to adjacent normal tissues. miR-155 directly up-regulates the m RNA and protein expression levels of β-catenin. In addition, miR-155 enhances cell invasion abilities, whereas the invasion potentiality was decreased after co-treated withβ-catenin si RNA. Conclusions： miR-155 promotes the invasion potential of colon cancer cell, at least partly through the upregulation of β-catenin. The findings of this study suggest that miR-155 and β-catenin may have a unique potential as a novel biomarker candidate for diagnosis and treatment in tumor metastasis.

关 键 词：结肠癌 MIR-155 Β-连环蛋白 侵袭 

分 类 号：R-33[医药卫生] R735.35