超声波辅助木瓜蛋白酶降解壳聚糖研究  

Study on Degradation of Chitosan by Papain under the Ultrasonic

作　　者：张翠玲[1] 贲爱玲[1] 张凤[1] Zhang Culling;Ben Ailing;Zhang Feng(Nanjing Xiao Zhuang University,Jiangsu Nanjing 211171)

机构地区：[1]南京晓庄学院,江苏南京211171

出　　处：《化工时刊》2018年第7期6-8,共3页Chemical Industry Times

基　　金：南京晓庄学院青年专项;项目编号为2011NXY68

摘　　要：讨论了超声波辅助木瓜蛋白酶降解壳聚糖的方法,研究了酶底比、反应温度、反应时间和溶液pH值对壳聚糖降解的影响。通过正交实验确定了超声波辅助木瓜蛋白酶降解壳聚糖的最佳条件为超声波功率120W,反应温度70℃,pH值5,降解时间30 min,酶底比1%。A degradation method of chitosan by papain under the ultrasonic was discussed. The effects of the ratio of enzyme to substrate,degradation temperature,degradation time and pH value on the degradation process of chitosan were investigated. Through the orthogonal experimental design,the optimum degradation conditions were： 120 W ultrasonic power,reaction temperature at 70 ℃ with pH value 5,degradation time of 30 min,1% ratio of enzyme to substrate.

关 键 词：超声波 壳聚糖 木瓜蛋白酶 降解 

分 类 号：O636.1[理学—高分子化学]