托盘根多糖初步纯化及其体外抗氧化活性研究  

The Preliminary Purification of Polysaccharide from Rubus Crategefolius Bunge. Root and Their Antioxidant Activity in Vitro

作　　者：杨秀东[1] 张嫔妹 张艳[1] 张扬[1] 崔浩[1] 周鸿立[1] YANG Xiu-dong;ZHANG Pin-mei;ZHANG Yan;ZHANG Yang;CUI Hao;ZHOU Hong-li(College of Chemical and Pharmaceutical Engineering,Jilin Institute of Chemical Technology-,Jilin City 132022,China;Library,Jilin Institute of Chemical Teehnology-,Jilin City 132022,China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022 [2]吉林化工学院图书馆,吉林吉林132022

出　　处：《吉林化工学院学报》2018年第7期90-94,共5页Journal of Jilin Institute of Chemical Technology

基　　金：吉林省科技发展计划项目(20170520039JH);吉林化工学院科学技术研究项目(2016-28);2017年吉林省大学生创新创业训练计划项目(702)

摘　　要：采用水提醇沉法提取托盘根多糖,粗多糖脱蛋白、透析后经DEAE-52纤维柱色谱分离纯化得到2个纯化多糖:RCP-1和RCP-2.通过体外清除DPPH、超氧阴离子和ABTS自由基评价纯化多糖的体外抗氧化活性.结果表明:RCP-1和RCP-2多糖含量分别为80.66%和82.15%.体外抗氧化实验表明,托盘根纯化多糖RCP-1和RCP-2在实验浓度范围内对DPPH、超氧阴离子和ABTS自由基均有一定的清除作用,同时具有良好的浓度依赖关系,其中RCP-2的自由基清除作用明显高于RCP-1.说明托盘根多糖具有较好的抗氧化活性,可能是其水溶性成分生物活性的物质基础.The crude polysaccharide of Rubus crategefolius Bunge. root was extract using water-extract and ethanol-precipitation method. It was further purified through de-protein and dialysis as well as DEAE-52 cellulose column chromatography. Two purified polysaccharide,RCP-1 and RCP-2 were obtained. The in vitro antioxidant activity of RCP-1 and RCP-2 was determined by DPPH,superoxide anion and ABTS scavenging assays.The results showed that the polysaccharide content of RCP-1 and RCP-2 were 80. 66% and 82. 15%,repectively.RCP-1 and PCP-2 had scavenging effect on DPPH,ABTS and superoxide anion. It also showed the concentration-dependent relationship.RCP-2 showed higher antioxidant activity that RCP-1.The results indicated that polysaccharide from Rubus crategefolius Bunge. root have a good antioxidant activity. It might be the material basis of the bioactivity of water soluble component of Rubus crategefolius Bunge.root.

关 键 词：托盘根 多糖 分离纯化 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]