癌胚抗原ssDNA核酸适配子的体外筛选及鉴定  

Screening and verification of ssDNA aptamers for carcinoembryonic antigen in vitro

作　　者：邢福军[1] 查成喜[1] 王小萍[1] 韩跃武[2] XING Fujun;ZHA Chengxi;WANG Xiaoping;HAN Yuewu(Traditional Chinese Medicineo f Gansu Provincial Hospital,Lanzhou,Gansu 730050,China;Lanzhou University,Lanzhou,Gansu 730000,China)

机构地区：[1]甘肃省中医院,兰州730050 [2]兰州大学,兰州730000

出　　处：《国际检验医学杂志》2018年第14期1694-1697,共4页International Journal of Laboratory Medicine

基　　金：甘肃省卫生行业科研计划项目(GSWST2013-07)

摘　　要：目的通过SELEX技术筛选获得癌胚抗原(CEA)特异性核酸适配子,为建立CEA适配子检测方法和肿瘤靶向治疗奠定实验基础。方法构建长度为88bp的随机ssDNA文库,体外筛选人血清CEA特异性适配子,十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)检测特异性适配子结合蛋白。结果经过优化体外筛选条件,经12轮筛选,获得特异性核酸适配子,经SDS-PAGE证实了其识别并特异性结合的物质为人血清CEA。结论经体外12轮筛选,证实了所获得的CEA适配子可特异性地与人血清CEA蛋白结合,为后续建立CEA特异性检测平台提供基础,也为靶向治疗药物的研制提供一定的思路。Objective To obtain specific aptamers of carcinoembryonic antigen（CEA）by SELEX in order to lay an exper sodium dodecyl sulfate polyacrylamide gel electrophoresis imental foundation for the establishment of CEA aptamer detection and tumor targeting therapy.Methods A random ssDNA library with the length of 88 bp was constructed to screen specific aptamers of CEA in vitro,and sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）was used to detect specific aptamer binding proteins.ResultsSpecific aptamers were obtained after 12 rounds of screening by optimizing screening conditions,and the specific binding substance was human serum CEA which was confirmed by SDS-PAGE.Conclusion Aptamers,that bind human serum CEA specifically,can be used to establish CEA specific detection platform and design targeting drugs.

关 键 词：癌胚抗原 SSDNA 检测 靶向治疗 

分 类 号：R446.6[医药卫生—诊断学]