云南高校图书馆联盟文献共享服务平台- SSDNA

SSDNA: 作品数：78被引量：226H指数：6; 导出分析报告; 相关领域：生物学更多>>; 相关作者：张印则韩跃武徐华吴凡梁延连更多>>; 相关机构：江南大学中国科学院广西科学院武汉大学更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划深圳市科技计划项目中央高校基本科研业务费专项资金更多>>

尿素变性PAGE中ssDNA回收方法的研究与优化: 《工业微生物》2025年第1期110-115,124,共7页郑楚君黄海轩王雨萱叶煜玮徐斐袁敏; 国家重点研发计划“食品营养与安全关键技术研发”重点专项(编号:2022YFF1101102)。; 从变性PAGE中获得高纯度、高回收率的ss DNA,是功能核酸研究及适配体筛选的关键环节之一。文章采用直取法、改良煮沸法、试剂盒回收和正丁醇回收4种方法,在尿素变性PAGE中对ss DNA进行了提取回收研究,并发现改良煮沸法的ss DNA回收效率...; 关键词：尿素变性PAGE ss DNA回收改良煮沸法

Translocation of ssDNA through Charged Graphene Nanopores:Effect of the Charge Density: 《Chinese Journal of Polymer Science》2024年第12期2048-2058,I0013,共12页Yuan-Shuo Zhang Zhi-Ya Qi Ming-Ming Ding Ming-Lun Li Tong-Fei Shi; This work was financially supported by the National Natural Science Foundation of China(Nos.22373025 and 22227804).; Nanopore sequencing harnesses changes in ionic current as nucleotides traverse a nanopore,enabling real-time decoding of DNA/RNA sequences.The instruments for the dynamic behavior of substances in the nanopore on the ...; 关键词：Nanopore sequencing Graphene nanoslit DNA translocation Electroosmotic flow

食源性致病菌ssDNA适配体的筛选及应用进展: 《食品与生物技术学报》2024年第11期44-59,共16页张明尹珍珍吴恒志; 广东省普通高校特色创新项目(2023KTSCX202);广东省教育科学规划课题(高等教育专项)(2024GXJK460)。; 单链DNA(single-stranded DNA,ssDNA)适配体是通过体外配体指数富集系统进化筛选获得的较短的单链脱氧核糖核苷酸序列。它们能够通过构象变化实现与靶标高亲和力和特异性结合,具有制备简单、成本低、稳定性高、易于修饰和免疫原性低等优...; 关键词：ssDNA适配体食源性致病菌筛选应用

A novel fluorescent structure-switching aptasensor for the sensitive detection of acrylamide based on AuNPs-assisted separation of ssDNA: 《Advanced Agrochem》2023年第3期276-283,共8页Ningyi Xu Xinyue Ma Yue Cao Huan Wang Han Wu Hongru Zheng Chuanyu Yang Ruiqi Zou Jiayi Liu Chunyan Sun Yuan Yuan; supported by Science and Technology Development Program of Jilin Province,China(No.20210509024RQ);On-budget Capital Construction Fund of Jilin Province Development and Reform Commission,China(2023C014);Graduate Innovation Fund of Jilin University,China(No.2022051);College Students'Innovative Entrepreneurial Training Plan Program of Jilin University,China(S202210183292).; Fluorescent aptasensor was developed for acrylamide(AAm)detection by utilizing the adsorption effect of Au nanoparticles(AuNPs)and fluorescence properties of SYBR Green I(SGI)towards double-stranded DNA(dsDNA).Compare...; 关键词：APTAMER ACRYLAMIDE Au nanoparticles SEPARATION Fluorescence

Cryo-EM structure of the ssDNA-activated SPARTA complex: 《Cell Research》2023年第9期731-734,共4页Minghui Guo Yuwei Zhu Zhiying Lin Dehui Yang Anqi Zhang Changyou Guo Zhiwei Huang; Dear Editor,Argonaute(Ago)proteins represent a highly conserved gene family,and are found in~10%bacteria,~30%archaea and almost all eukaryotes.l The eukaryotic Ago(eAgo)proteins are the core element of RNA-induced sil...; 关键词：SPAR TRANSLATIONAL COMPLEX

基于氧化石墨烯荧光传感技术对中药海马的鉴定被引量：1: 《中国实验方剂学杂志》2023年第6期185-193,共9页钟洪金任九卓林张灵迂侯飞侠; 国家自然科学基金项目(81903757)。; 目的:建立基于氧化石墨烯荧光传感技术鉴定中药海马的方法,为中药鉴定提供一个新的研究思路。方法:在小海马特异性上游引物Ja-F的5’端标记荧光基团FAM作为核酸探针FAM-单链DNA(ssDNA),在下游引物Ja-R的5’端加上RNA聚合酶Ⅱ的识别位点...; 关键词：氧化石墨烯荧光传感技术核酸探针海马鉴定单链DNA(ssDNA)

ssDNA functionalized nanodiamonds for uranium decorporation: 《Chinese Chemical Letters》2022年第7期3570-3572,共3页Qinglong Yan Yu Miao Xiaomei Wang Jifei Ma Juan Diwu Ying Zhu Shuao Wang Chunhai Fan; supported by grants from the National Natural Science Foundation of China(Nos.82050005,21976127,22022410);the Youth Innovation Promotion Association of CAS(No.2016236);the Natural Science Foundation of Jiangsu Province(Nos.BK20190044,BK20210736);funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD);the China Postdoctoral Science Foundation(No.2020M681431)。; The hunt for agents that are suitable for actinide decorporation to reduce the whole-body load of actinide in accidental internal exposure is the ever-lasting goal in radiation protection and medical treatment in nucl...; 关键词：NANODIAMOND SSDNA URANIUM DECORPORATION

针对RhD血型抗原的单链寡核苷酸适配体修饰红细胞毒性的初步研究被引量：1: 《中国输血杂志》2022年第5期484-487,共4页李璐张印则王斌王铸; 国家自然科学基金(81802566);深圳市龙华区科技创新基金项目(2020013、2020003);深圳市南山区卫生科技项目(NS2021164)。; 目的探讨RhD血型抗原的单链寡核苷酸(ssDNA)适配体对红细胞毒性的影响。方法通过基因合成得到的2条全长82 bp ssDNA的RhD血型抗原适配体;收集5份EDTA抗凝全血,分别制备成4×10^(7)/mL的悬浮红细胞,均分为10管,各5管(100μL/管)标记为...; 关键词：单链寡核苷酸(ssDNA) 适配体(NAA) RhD血型抗原细胞毒性

Oxidation degree dependent adsorption of ssDNA onto graphene-based surface: 《Chinese Physics B》2021年第10期488-494,共7页Huishu Ma Jige Chen Haiping Fang Xiaoling Lei; supported by the National Natural Science Foundation of China(Grant Nos.11305237 and 11974366);the Fundamental Research Funds for the Central Universities,China,the Natural Science Foundation of Shanghai,China(Grant No.19ZR1463200);the Key Research Program of Chinese Academy of Sciences(Grant No.QYZDJ-SSW-SLH053).; DNA/GO composite plays a significant role in the research field of biotechnology and nanotechnology,and attracts a great deal of interest.However,it is still unclear how the oxidation degree of the graphene-based surf...; 关键词：single-strand DNA(ssDNA) molecular dynamics simulation oxidation degrees graphene-based surfaces

A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630被引量：2: 《Synthetic and Systems Biotechnology》2021年第3期200-208,共9页Youxiang Liang Yuwen Wei Song Jiao Huimin Yu; supported by the National Key R&D Program of China(2018YFA0902200);Natural Science Foundation of China(No.21776157,No.22078173)。; Genome engineering of Rhodococcus opacus PD630,an important microorganism used for the bioconversion of lignin,is currently dependent on inefficient homologous recombination.Although a CRISPR interference procedure fo...; 关键词：Rhodococcus opacus CRISPR/Cas9 ssDNA recombineering Novel recombinase Genome editing

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