食源性致病菌ssDNA适配体的筛选及应用进展  

Progress on Screening and Application of ssDNA Aptamers for Foodborne Pathogens

作　　者：张明 尹珍珍 吴恒志 ZHANG Ming;YIN Zhenzhen;WU Hengzhi(School of Yunkang Medicine and Health,Nanfang College,Guangzhou 510970,China;Biomedical Big Data Center,Nanfang College,Guangzhou 510970,China)

机构地区：[1]广州南方学院云康医学与健康学院,广东广州510970 [2]广州南方学院生物医学大数据中心,广东广州510970

出　　处：《食品与生物技术学报》2024年第11期44-59,共16页Journal of Food Science and Biotechnology

基　　金：广东省普通高校特色创新项目(2023KTSCX202);广东省教育科学规划课题(高等教育专项)(2024GXJK460)。

摘　　要：单链DNA(single-stranded DNA,ssDNA)适配体是通过体外配体指数富集系统进化筛选获得的较短的单链脱氧核糖核苷酸序列。它们能够通过构象变化实现与靶标高亲和力和特异性结合,具有制备简单、成本低、稳定性高、易于修饰和免疫原性低等优点,因此被称为“化学抗体”。ssDNA适配体已在食品安全检测领域显示出巨大潜力。作者综述了基于细胞指数富集配体系统进化(systematic evolution of ligands by exponential enrichment,SELEX)技术发展的ssDNA适配体筛选技术,以及ssDNA适配体在食源性致病菌检测领域中的应用,总结了目前研究的不足以及未来发展方向,旨在为提高食品安全监管与食源性疾病预防和控制效果提供理论依据。Single-stranded DNA(ssDNA)aptamer is a short single-stranded deoxyribonucleotide sequence obtained by the systematic evolution of ligands by exponential enrichment(SELEX)in vitro.It can achieve high affinity and specific binding to targets through conformational changes and possesses the advantages of simple preparation,low cost,relative stability,ease of modification,and low immunogenicity,earning it the name“chemical antibodies”.ssDNA aptamers have shown great potential in the field of food safety detection.This paper reviewed the ssDNA aptamer screening technology based on SELEX,its application in the detection of foodborne pathogens,current research limitations and potential future development directions.The aim is to provide a theoretical basis for improving food safety supervision and the prevention and control of foodborne disease.

关 键 词：ssDNA适配体 食源性致病菌 筛选 应用 

分 类 号：O64[理学—物理化学]