A novel fluorescent structure-switching aptasensor for the sensitive detection of acrylamide based on AuNPs-assisted separation of ssDNA  

作　　者：Ningyi Xu Xinyue Ma Yue Cao Huan Wang Han Wu Hongru Zheng Chuanyu Yang Ruiqi Zou Jiayi Liu Chunyan Sun Yuan Yuan 

机构地区：[1]Department of Food Quality and Safety,College of Food Science and Engineering,Jilin University,Changchun,130062,China [2]Jilin Province Product Quality Supervision and Inspection Institute,Changchun,130103,China

出　　处：《Advanced Agrochem》2023年第3期276-283,共8页现代农业化学（英文）

基　　金：supported by Science and Technology Development Program of Jilin Province,China(No.20210509024RQ);On-budget Capital Construction Fund of Jilin Province Development and Reform Commission,China(2023C014);Graduate Innovation Fund of Jilin University,China(No.2022051);College Students'Innovative Entrepreneurial Training Plan Program of Jilin University,China(S202210183292).

摘　　要：Fluorescent aptasensor was developed for acrylamide(AAm)detection by utilizing the adsorption effect of Au nanoparticles(AuNPs)and fluorescence properties of SYBR Green I(SGI)towards double-stranded DNA(dsDNA).Compared to the binding of aptamer with AAm,the higher affinity of aptamer with cDNA may facilitate a structure switching from the aptamer/AAm complex to aptamer/cDNA dsDNA.The free aptamers were adsorbed onto AuNPs and separated by centrifugation.Subsequently,SGI was introduced as a fluorescent reporter for quantitative detection.Compared to conventional AuNPs-based colorimetric detection,the sensitivity of this strategy was improved by 3.18-fold in the range of 0.005–50 mg/L with a low detection limit of 4.68μg/L.The method has been successfully applied to analyze fried twists and biscuits.Notably,it is a low-cost and general method that provides guidance for the development of rapid screening technology in the field.

关 键 词：APTAMER ACRYLAMIDE Au nanoparticles SEPARATION Fluorescence 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]