异氟烷对体外精子超微结构的影响  

Effects of Isoflurane on Ultrastructure of Human Sperm in Vitro

作　　者：郑立宏[1] 杨丹[2] 高晓勤[3] 丁贤胜 孙发[5] ZHENG Lihong;YANG Dan;GAO Xiaoqin;DING Xiansheng;SUN Fa(Guiyang Nursing Vocational College,Guiyang 550081,Guizhou,China;Surgery Department of Guizhou Medical University,Guiyang 550004,Guizhou,China;Zunyi Medical and Pharmaceutical College,Zunyi 563002,Guizhou,China;People ＇s Hospital of Liupanshui,Liupanshui 553001,Guizhou,China;Urology Department of the AffiliatedHospital of Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵阳护理职业学院,贵州贵阳550081 [2]贵州医科大学外科学教研室,贵州贵阳550004 [3]遵义医药高等专科学校,贵州遵义563002 [4]六盘水市人民医院,贵州六盘水553001 [5]贵州医科大学附院泌尿外科,贵州贵阳550004

出　　处：《贵州医科大学学报》2018年第7期754-756,共3页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81360097);贵州省科学技术厅研究项目[黔科合LH字(2014)7087]

摘　　要：目的:探讨异氟烷在体外对人类精子超微结构的影响。方法:收集24例精液常规正常的男性精子标本,每例精液均分成3份,分别给予1.1%异氟烷(A组)、2.2%异氟烷(B组)和空气(C组),并放置于孵箱培养5 h,采用扫描电镜观察各组精子的超微结构改变,计算异氟烷处理后顶体完整率、头部及尾部畸形率。结果:用异氟烷孵育后,头部及尾部畸形、顶体凹陷的精子数量增多;A、B组精子顶体完整率低于C组、头部及尾部畸形率高于C组,其中B组精子形态异常情况较A组更为严重,差异有统计学意义(P<0.05)。结论:异氟烷可影响精子形态,且随着异氟烷浓度增加,精子形态改变越来越明显。Objective： To investigate the effect of isoflurane on ultrastructure of human sperm in vitro. Methods： 24 normal male sperm samples were collected. Each was divided into three groups and placed respectively in three tubes： 1. 1% isoflurane（ group A）,2. 2% isoflurane（ group B） and air（ group C）. All of the tubes were placed in the incubator for 5 hours. The ultrastructural changes of sperms were observed by electron microscopy scanning to calculate the acrosome integrity rate,head and tail deformity rate. Results： After incubation with isoflurane,the number of sperms with head and tail deformity,and the acrosomatic depression increased. The sperm acrosome integrity rate in group A and B was lower than that in group C,and the head and tail deformity rate was higher than that in group C. The abnormal morphology of sperms in group B was more serious than that in group A. The difference was statistically significant（ P〈0. 05）. Conclusion： As the isoflurane concentration increases,changes of sperm morphology have become increasingly obvious with the integrity of sperm acrosome decreased and sperm deformity rate increased.

关 键 词：异氟烷 生殖功能 精子 超微结构 扫描电镜 

分 类 号：R699[医药卫生—泌尿科学]