IL-1B促进naive T细胞向Th22细胞分化及在非小细胞肺癌中表达的相关性研究  

IL-1B promotes differentiation of naive T cells into Th22 cells and its expression in non-small cell lung cancer

作　　者：杨毅 官俏兵 郭丽 韩晨阳 YANG Yi;GUAN Qiao-bing;GUO Li;HAN Chen-yang(The Second Hospital of Jiaxing,Jiaxing 314001,China.)

机构地区：[1]嘉兴市第二医院，浙江 嘉兴 314001

出　　处：《中国病理生理杂志》2018年第7期1301-1305,共5页Chinese Journal of Pathophysiology

摘　　要：目的:研究白细胞介素1β(IL-1β)促进nave T细胞向Th22细胞转化的机制及其在非小细胞肺癌患者外周血中表达的相关性和临床意义。方法:采用CD4^+nave T细胞磁珠分选试剂盒分离健康人外周血单个核细胞中的CD4^+nave T细胞,加入转化生长因子β和IL-2促进其分化增殖,分化过程中加入IL-1β诱导其向Th22细胞的分化,流式细胞术检测CD4^+IL-22^+T细胞的比例,ELISA检测IL-22的表达。选择我院确诊为非小细胞肺癌的患者60人,其中Ⅰ期18人,Ⅱ期20人,Ⅲ期13人,IV期9人,同时选择健康人25例,用流式细胞术检测外周血中Th22(CD4^+IL-22^+)细胞的比例,ELISA检测血清中IL-1β和IL-22的水平。结果:IL-1β可以诱导na6ve T细胞向Th22细胞转化并促进IL-22的分泌(P<0.05)。非小细胞肺癌患者外周血中Th22细胞比例及IL-22和IL-1β的水平均高于健康人且与临床分期相关(P<0.05)。结论:IL-1β可以诱导Th22细胞的分化和IL-22的表达,三者的水平和非小细胞肺癌的进展相关,可能参与免疫抑制并促进非小细胞肺癌的发生。AIM： To investigate the mechanism of interleukin-1β（ IL-1β） promoting the transformation of nave T cells into Th22 cells and the correlation of its peripheral blood expression in non-small cell lung cancer patients.METHODS： CD4~＋nave T cell magnetic bead sorting kit was used to isolate the peripheral blood mononuclear T cells from healthy people. Transforming growth factor-β（ TGF-β） and IL-2 were added to promote differentiation and proliferation. IL-1β was used to induce differentiation into Th22 cells. The proportion of CD4~＋IL-22~＋T cells was analyzed by flow cytometry,and the expression of IL-22 was detected by ELISA. We selected 60 cases of non-small cell lung cancer patients in our hospital,including 18 in Ⅰ phase,20 in Ⅱ phase,13 in Ⅲ phase and 9 in IV phase,as well as 25 healthy persons.The proportion of Th22（ CD4~＋IL-22~＋） cells in peripheral blood was detected by flow cytometry,and the serum levels of IL-1β and IL-22 were measured by ELISA. RESULTS： IL-1β induced the transformation of na6 ve T cells into Th22 cells and promoted the secretion of IL-22（ P〈0. 05）. The proportion of Th22 cells and the IL-22 and IL-1β levels in peripheral blood of the patients with non-small cell lung cancer were higher than those in healthy subjects,and correlated with the clinical stage. CONCLUSION： IL-1β induces the differentiation of Th22 cells and the expression of IL-22. The levels of IL-1β and IL-22 are related to the progression of non-small cell lung cancer,which may be involved in immunosuppression and promote the occurrence of non-small cell lung cancer.

关 键 词：白细胞介素1B NAIVE T细胞 Th22细胞 非小细胞肺癌 白细胞介素22 

分 类 号：R734.2[医药卫生—肿瘤] R363.2[医药卫生—临床医学]