大鼠背根神经节细胞中TRPV1与cAMP/PKA通路调控机制研究  

Study on regulatory mechanism of TRPV1 and cAMP/PKA pathway in dorsal root ganglion cells of rats

作　　者：高峰[1] 卢伟龙 王华林[1] 吴媛媛 王斌[1] 李敏[1] GAO Feng;LU Wei-long;WANG Hua-lin;WU Yuan-yuan;WANG Bin;LI Min(College of Pharmaceutical Science,Shanxi University of Chinese Medicine,Xi’ an 712000,China)

机构地区：[1]陕西中医药大学药学院,陕西西安712000

出　　处：《中国药理学通报》2018年第8期1110-1114,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81303243);陕西省中医药管理局项目(No JCPT007)

摘　　要：目的探讨大鼠背根神经节细胞中,辣椒素受体(transient receptor potential vanilloid 1,TRPV1)与体内cAMP/PKA信号通路的调控作用。方法分离培养大鼠背根神经节原代细胞,分别应用cAMP/PKA信号通路的激动剂8-Br-cAMP和抑制剂H-89,TRPV1的激动剂辣椒素(capsaicin)、抑制剂i RTX进行干预,应用定量PCR和Western blot检测各组细胞的TRPV1、p-PKA的基因和蛋白表达,探讨TRPV1与体内cAMP/PKA信号通路之间的调控机制。结果与空白对照组比较,8-Br-cAMP组、capsaicin组TRPV1的基因及蛋白表达均明显增强,iRTX组TRPV1的基因及蛋白表达明显降低,H-89组TRPV1基因、蛋白的表达无明显变化;8-Br-cAMP组p-PKA的基因及蛋白表达明显增强,H-89组p-PKA的基因及蛋白表达明显降低,而capsaicin组、iRTX组p-PKA基因、蛋白的表达无明显变化。结论 cAMP-PKA信号通路是调控TRPV1表达的重要作用机制之一,同时可能存在其他信号通路对TRPV1也有一定调控作用。Aim To investigate the regulation of TRPV1 and cAMP/PKA signal pathway in rat dorsal root ganglion（DRG） cells. Methods Primary cells of rat DRG were isolated and treated with 8-Br-cAMP and H-89,agonist and inhibitor of cAMP/PKA signaling pathway,capsaicin and i RTX,agonist and inhibitor of TRPV1. The expression of TRPV1 and p-PKA in each group of cells was detected by q PCR and Western blot.The regulatory mechanism of TRPV1 and cAMP/PKA signaling pathway was explored. Results Compared with the blank control group,the gene and protein expression of TRPV1 in capsaicin group significantly increased,while the gene and protein expression of TRPV1 in i RTX group significantly decreased. However,the expression of p-PKA gene and protein showed no significant change. The gene and protein expressions of p-PKA and TRPV1 in 8-Br-cAMP group were significantly enhanced,and the gene and protein expressions of p-PKA and TRPV1 in H-89 group significantly decreased. Conclusions The cAMP-PKA signaling pathway is one of the most important mechanisms regulating the expression of TRPV1. At the same time,other signaling pathways may have some regulatory roles in TRPV1.

关 键 词：大鼠背根神经节 原代细胞 TRPV1 cAMP/PKA通路 磷酸化 调控 

分 类 号：R-332[医药卫生] R322.8