外源性α1-抗胰蛋白酶对非小细胞肺癌转移与侵袭的促进作用研究  

Effect of Exogenous Alpha 1-antitrypsin on the Promotion of Metastasis and Invasion of Non-small Cell Lung Cancer

作　　者：杨敏[1] 李奕 吴东明[1] 刘腾[1] 刘明全[1] 许颖[1] Yang Min;Li Yi;Wu Dongming;Liu Teng;Liu Mingquan;Xu Ying(Clinical Laboratory,The First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,China;Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院第一附属医院检验科,成都610500 [2]成都医学院,成都610500

出　　处：《成都医学院学报》2018年第3期270-273,338,共5页Journal of Chengdu Medical College

基　　金：四川省教育厅高校科研创新团队资助项目(No:17TD0012);成都医学院科研创新团队资助项目(No:CYTD15-03)

摘　　要：目的探讨α1-抗胰蛋白酶(A1AT)对非小细胞肺癌(NSCLC)转移与侵袭的促进作用。方法向细胞培养液中加入不同质量浓度A1AT(0,600,1 200,2 400ng/mL),采用免疫印迹法和免疫荧光技术分别检测上皮细胞标志物E-cadherin、间质细胞标志物N-cadherin、Vimentin;采用细胞transwell实验和划痕试验检测细胞的转移和侵袭能力。结果 A1AT质量浓度越高,E-cadherin表达越低,N-cadherin和Vimentin表达含量越高,细胞的转移和侵袭能力越强。结论外源性A1AT能够促进NSCLC的转移与侵袭。Objective To investigate the effect of alpha 1-antitrypsin （A1AT） on the promotion of metastasis and invasion of non-small cell lung cancer （NSCLC）. Methods The different concentrations of A1AT （0, 600, 1200, 2400ng/ml） were added into the cell culture solution.Western blot and immunofluorescence were used to detect the epithelial cell marker E-cadherin and the mesenchymal markers including N-cadherin and Vimentin.The transwell and wound-healing assays were used to detect the ability of the cell metastasis and invasion. Results The expression of E-cadherin was decreased with the increase of A1AT concentration, and the ability of cell metastasis and invasion was better with the increasing expression of N-cadherin and Vimentin. Conclusion Exogenous A1AT promotes the metastasis and invasion of non-small cell lung cancer.

关 键 词：Α1-抗胰蛋白酶 非小细胞肺癌 上皮间质转化 

分 类 号：R73-37[医药卫生—肿瘤]