夫拉平度对人宫颈癌CasKi细胞裸鼠皮下种植瘤生长的抑制作用研究  

Research on Inhibition Effect of Flavopiridol on the Growth of in Human Cervical Carcinoma Cas Ki Subcutaneous Implanted in Nude Mice

作　　者：周焕芹[1] ZHOU Huan-qin(Department of Pharmacy, Liaocheng People＇ s Hospital, Liaoeheng, Shandong Province, 252000 China)

机构地区：[1]山东省聊城市人民医院药学部,山东聊城252000

出　　处：《中外医疗》2018年第12期18-21,共4页China & Foreign Medical Treatment

摘　　要：目的研究周期蛋白激酶(CDKs)抑制剂夫拉平度(Flavopiridol,FP)对人宫颈癌Cas Ki细胞裸鼠皮下移植瘤生长和转移的影响。方法 2017年7—8月在聊城市人民医院中心实验室建立Cas Ki细胞裸鼠模型,随机分为对照组、顺铂组、FP组及FP和顺铂联合用药组,每组8只裸鼠,4组均于肿瘤抑制成功后瘤体直径平均为0.8 cm开始给药,给药3周,2次/周。最后一次给药5 d后处死全部裸鼠,测量移植瘤的体积和重量,采用免疫印迹法检测对各组中肿瘤组织凋亡蛋白Caspase 3表达的影响,并采用分光光度法测定裸鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果与对照组相比[(567.02±89.38)V/mm3;(0.63±0.07)m/g],顺铂组[(114.50±18.07)V/mm3,(0.12±0.04)m/g]、FP组[(106.74±21.39)V/mm3;(0.10±0.02)m/g]及FP和顺铂联合用药组[(88.59±19.84)V/mm3,(0.07±0.02)m/g]荷瘤体积和重量明显减小(P<0.05);顺铂组、FP组及FP和顺铂联合用药组Caspase 3表达均显著增加(P<0.05);与对照组相比[(134.68±45.63)U/mL,(3.45±1.47)mmol/L],顺铂组[(143.72±44.45)U/mL,(2.76±2.83)mmol/L]、FP组[(156.51±32.53)U/mL,(2.64±0.66)mmol/L]及FP和顺铂联合用药组[(208.37±63.35)U/mL,(2.38±1.13)mmol/L]血清SOD的活性显著升高(P<0.05),MDA的含量显著降低(P<0.05)。结论夫拉平度对人宫颈癌Cas Ki细胞皮下种植瘤生长具有抑制作用,且与促进癌细胞的凋亡,抑制机体氧化应激反应有关。Objective To study the effect of flavopiridol（FP） on human cervical carcinoma subcutaneous implanted tumor growth inhibition in mice bearing Cas Ki cell. Methods The nude rat model of cervical cancer cell was established from July to August 2017 in Central laboratory of Liaocheng People＇s Hospital. The nude rat were randomly divided into four groups（control group, cisplatin group, FP group and combined treatment group, 8 rats in each group）, and the four groups were administered when the average tumor diameter was 0.8 cm, 3 w, and 2 times/w, and all the mice were sacrificed in 5 days after the adminitration, and the volume and weight of tumors were tested. And the expression of caspase3 was determined by immunoblotting. And the SOD and MDA levels were measured by the spectrophotometry. Results The tumor volume and weight obviously decreased in the cisplatin group [（114.50±18.07）V/mm3;（0.12±0.04）m/g], FP group [（106.74±21.39）V/mm3;（0.10±0.02）m/g] and combined treatment group [（88.59±19.84）V/mm3;（0.07±0.02）m/g]（P 0.05） compared with that in the control group[（567.02±89.38）V/mm3;（0.63±0.07）m/g）], and the expression of caspase 3 in the cisplatin group, FP group and combined treatment group obviously increased compared with that in the control group [（134.68±45.63）U/mL,（3.45±1.47）mmol/L]. The activition of serum SOD in the cisplatin group[（143.72±44.45）U/mL,（2.76 ±2.83）mmol/L], FP group[（156.51±32.53）U/mL,（2.64±0.66）mmol/L] and combined treatment group [（208.37±63.35）U/mL,（2.38±1.13）mmol/L] obviously increased（P0.05）, the contents of MDA obviously decreased（P0.05）. Conclusion FP has an inhibition effect on the growth of in human cervical carcinoma Cas Ki subcutaneous implanted in nude mice, which is related to promoting the apoptosis of cancer cells and inhibiting the oxidative stress reaction of the body.

关 键 词：夫拉平度 顺铂 宫颈癌 生长抑制 凋亡 氧化应激 

分 类 号：R73[医药卫生—肿瘤]