黏蛋白-4在膀胱癌中的表达及作用机制  

Expression of mucin-4 in bladder cancer and its mechanism

作　　者：刘远[1] 劳辉恒 李宇同[2] LIU Yuan;LAO Huihen;LI Yutong(Department of Urinary Surgery,Central Hospital of Kaiping in Guangdong,Kaiping 529300,China;Department of Urinary Surgery,The First Affiliated Hospital of Jinan University,Guangzhou 510507,China)

机构地区：[1]广东省开平市中心医院泌尿外科,广东开平529300 [2]暨南大学附属第一医院泌尿外科,广东广州510507

出　　处：《分子影像学杂志》2018年第3期354-357,共4页Journal of Molecular Imaging

摘　　要：目的探讨黏蛋白-4(mucin-4)在膀胱癌中的表达及作用机制。方法选取广东省两家三甲医院2016年1月~2017年6月期间收集的膀胱癌标本115例,同时选取距离肿瘤边缘>2 cm的癌旁组织作为对照。SYBR green实时荧光定量PCR法和Western blot检测mucin-4在膀胱癌、癌旁组织中的相对表达量,并分析mucin-4与临床相关参数的关系,同时观察mucin-4过对T24细胞(膀胱癌细胞系)增殖、凋亡的影响。结果膀胱癌组织mucin-4的相对表达水平高于正常膀胱组织,差异具有统计学意义(P<0.05)。mucin-4的表达水平与膀胱癌的肿瘤大小、有无淋巴结转移以及TNM分期有关(P<0.05);肿瘤直径大于5 cm组mucin-4的表达水平高于肿瘤直径小于5 cm组;有淋巴结转移的膀胱癌组mucin-4的表达水平高于无淋巴结转移的膀胱癌组;Ⅲ+Ⅳ期膀胱癌组mucin-4的表达水平明显高于Ⅰ+Ⅱ期膀胱癌,差异具有统计学意义(P<0.05)。mucin-4的表达水平与膀胱癌患者的年龄、性别无关(P>0.05)。上调mucin-4的表达,可以诱导膀胱癌细胞(T24细胞系)的增殖,抑制凋亡;下调mucin-4的表达,可以诱导膀胱癌细胞(T24细胞系)的凋亡,抑制增殖。结论 mucin-4在膀胱癌的发生发展中起着重要作用,其表达水平对膀胱癌的严重程度及预后情况具有一定的参考价值。Objective To study the expression of mucin-4 in bladder cancer and its mechanism. Methods A total of 120 cases of bladder cancer admitted to our hospital from January 2015 to December 2016 were selected as experimental group and adjacent tissues 2 cm around the tumor were selected as the control group. The relative expression of mucin-4 in bladder cancer and adjacent tissues was analyzed by SYBR green real-time quantitative PCR and western blot test. The relative expression of mucin-4 in bladder cancer carcinoma was analyzed and the relationship between mucin-4 and clinical features was analyzed.Results The relative expression level of mucin-4 in bladder cancer tissues was significantly higher than that in normal bladder tissues（P 0.05）. The expression level of mucin-4 was associated with tumor size, lymph node metastasis, and TNM stage of bladder cancer（P 0.05）. The expression level of mucin-4 in tumor tissues with diameter greater than 5 cm was higher than that in tumor group with diameter less than 5 cm. The expression level of mucin-4 in bladder transition group was higher than that in bladder transition group without lymph node metastasis. The expression level of mucin-4 in bladder tumor at III ＋ IV stage was significantly higher than that at I ＋ II stage（P 0.05）. The expression level of mucin-4 was not related to the age and sex of bladder cancer patients（P〉0.05）. Mucin-4 overexpression induced the proliferation of bladder cancer cells（T24 cell line） and inhibited apoptosis, while mucin-4 knockout inhibited the proliferation of bladder cancer cells（T24 cell line） and induced apoptosis. It is suggested that mucin-4 can promote the proliferation of bladder cancer cells and inhibit apoptosis. Conclusion Mucin-4 plays an important role in the development and progression of bladder cancer, and its expression level has certain reference value for the severity and prognosis of bladder cancer.

关 键 词：膀胱癌 黏蛋白-4 转移 

分 类 号：R737.14[医药卫生—肿瘤]