免疫组化法和PCR法检测LMP-1筛查NPC的辅助诊断效果比较  

作　　者：龚梁[1] 陈亮宇[1] 熊华[1] 陈建强[1] 邹坚定[1] 鲁杰[1] 

机构地区：[1]浙江省慈溪市人民医院,315300

出　　处：《浙江临床医学》2018年第9期1565-1566,共2页Zhejiang Clinical Medical Journal

基　　金：浙江省自然科学基金（LY12H13002）;浙江省医药卫生科技计划（2015RCB026）;宁波市自然科学基金（2012A610205、2012A610206）;浙江省中医药科技计划项目（2015ZB107）

摘　　要：目的采用免疫组化法和聚合酶链反应（PCR）法检测EB病毒潜伏膜蛋白1（LMP-1），比较两种方法筛查鼻咽癌（NPC）的效果。方法采用免疫组化法和pcR法检测NPc鼻咽标本LMP—1表达，采用受试者工作曲线（ROC）分析两种方法诊断筛查NPc的效果。结果LMP-1免疫组化筛查鼻咽癌的灵敏度为0．633，特异度为0．903，准确度为0．780；LMP—1PCR筛查鼻咽癌的灵敏度为0．933，特异度为0．875，准确度为0．902；LMP-1PCR法的ROC曲线下面积为0．904，明显高于LMP-1免疫组化法0．768（P〈005）。结论鼻咽标本的LMP-1PCR法筛查NPC，其诊断效果明显好于LMP-1免疫组化法，且具有较高的灵敏度和特异度，值得临床推广应用。Objective To compare the immunohistochemical method and polymerase chain reaction （ PCR ） method for detection of EB latent membrane protein 1 （ LMP-I ） in screening nasopharyngeal carcinoma （ NPC ） . Methods The immunohistochemical method and PCR method were used to detected LMP-1 expression of NPC tissue, and ROC method was used to analyze diagnostic effect of the two methods in screening NPC. Results The sensitivity, specificity and accuracy of LMP-1 by immunohistochemical method screening NPC were respectively 0.633, 0.903 and 0.780, and LMP-1 by PCR method were respectively 0.933, 0.875 and 0.902. Area under ROC of LMP-1 PCR method was 0.904, much higher than LMP-1 immunohistochemical method （P〈0.05） . Conclusion The diagnostic effect of LMP-I PCR method of nasopharyngeal swab screening NPC is much better than LMP-1 immunohistochemical method, and PCR method has high sensitivity and specificity. It deserves to spread in clinical.

关 键 词：EB病毒 鼻咽癌 潜伏膜蛋白1 免疫组化 聚合酶链反应 诊断试验 

分 类 号：R733.1[医药卫生—肿瘤]