GC-MS结合AMDIS及Kováts保留指数研究不同虫蛀程度陈皮挥发性成分变化规律  

Variation of volatile components in Citri Reticulatae Pericarpium after attack of insects by GC-MS combined with AMDIS and Kováts retention index

作　　者：王智磊[1] 伍清芳 刘素娟[1] 张鑫[1] 陈林[1] 陈鸿平[1] 刘友平[1] WANG Zhi-lei;WU Qing-fang;LIU Su-juan;ZHANG Xin;CHEN Lin;CHEN Hong-ping;LIU You-ping(Pharmacy College,Chengdu University of Chinese Medicine,Key Laboratory of Quality Standardization of Chinese Medicinal Materials,Breeding Base and Key Laboratory of Systematic Research and Development of Traditional Chinese Medicine Resources at the Provincial and Ministerial Level,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院,中药材质量标准化教育部重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中华中医药杂志》2018年第8期3327-3331,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81072991)

摘　　要：目的:研究不同虫蛀程度陈皮挥发油的化学成分,并比较不同虫蛀程度陈皮挥发性成分的差异。方法:采用水蒸气蒸馏法提取陈皮挥发油,计算挥发油得率并进行比较。气相色谱-质谱联用技术(GC-MS)结合自动质谱退卷积定性系统(AMDIS)及Kováts保留指数分析其挥发性成分,面积归一化法比较各组分间的相对含量。结果:不同虫蛀程度陈皮挥发油含量分别为4.0%、6.0%和3.6%,共分离得到28个挥发性成分,分别占总挥发性成分的97.979%、95.959%、94.385%,挥发油的主要类型为单萜烯、倍半萜烯以及低分子量的酚、醛、醇。3个样品中共有挥发性成分17个,相对含量均大于1.0%的成分从高到低依次为:D-柠檬烯>γ-萜品烯>β-月桂烯。结论:陈皮经虫蛀后挥发油得率发生明显改变,不同虫蛀程度陈皮挥发性成分种类和含量存在明显差异,为阐明虫蛀对陈皮品质的影响提供理论依据。Objective： To study the chemical constituents of the volatile oil from the different samples after insects attack and compare the differences of the volatile components of Citri Reticulatae Pericarpium （CRP）. Methods： Volatile oil was extracted by steam distillation, and the yield of volatile oil was calculated and compared. Gas Chromatography-Mass Spectrometer （GC-MS） combined with Automated Mass Spectral Deconvolution ＆ Identification System （AMDIS） and Kov^ts retention index were used to analyze the volatile components. The relative content of each component was compared. Results： The contents of volatile oil were 4.0%, 6.0% and 3.6%, respectively in different samples and 28 volatile components were isolated accounting for the total volatile components 97.979%, 95.959% and 94.385% respectively. The main types of volatile oil were monoterpenes, sesquiterpenes, and low molecular weight phenols, aldehydes and alcohols. Among the three samples, there were 17 volatile components and the components whose relative content is greater than 1.0% from high to low were D-limonene〉veterpinene〉 ermyrcene respectively. Conclusion： The yield of volatile oil was significantly changed after insects attack. The content of the volatile components from the different insects CRP samples were different, which provided the theoretical basis for clarifying the quality of CRP after attack of insects.

关 键 词：陈皮 虫蛀 气相色谱-质谱联用技术 自动质谱退卷积定性系统 保留指数 挥发油 

分 类 号：R284.1[医药卫生—中药学]