邻苯二甲酸酯高效降解菌的筛选及表征  

Screening and Characterization of an Efficient Phthalate-Ester-Degrading Strain

作　　者：杨捷[1] 姚炎华 尹大强[2] 叶秀云[1] YANG Jie;YA;YINDaqiang;YEXiuyun(Fujian Key Laboratory of Marine Enzyme Engineering,Fuzhou University,Fuzhou 350116,China;The Yangtze River Water Environment Key Laboratory of the Ministry of Education,TongjiUniversity,Shanghai 200092,China)

机构地区：[1]福州大学福建省海洋酶工程重点实验室,福建福州350116 [2]同济大学长江水环境教育部重点实验室,上海200092

出　　处：《同济大学学报（自然科学版）》2018年第7期944-950,共7页Journal of Tongji University:Natural Science

基　　金：国家自然科学基金(31671795;41306120);海洋生物酶工程创新服务平台(2014FJPT02)

摘　　要：以邻苯二甲酸二丁酯(DBP)为唯一碳源,筛选得到一株能够降解DBP的菌株.通过形态学观察、16SrDNA测序及系统发育分析,鉴定该菌株为寡养单胞菌(Stenotrophomonas sp.),命名为B3.首次报道了寡养单胞菌对邻苯二甲酸酯(PAEs)的降解作用.B3可高效降解0.05~50g·L^(-1)浓度范围的DBP,其中对0.05g·L^(-1) DBP的降解率为93.4%.经过优化后,在35℃、pH 8条件下对10g·L^(-1) DBP的降解率为95.8%.反应动力学研究表明,B3降解DBP符合一级降解动力学模型,对50g·L^(-1) DBP初始降解速率可达1.25g·L^(-1)·h-1.B3对其他4种常见的PAEs(邻苯二甲酸二(2─乙基己)酯、邻苯二甲酸二甲酯、邻苯二甲酸丁苄酯、邻苯二甲酸二乙酯)和苯胺、甲苯、邻苯二甲酸的降解率均在50%及以上.B3降解PAEs浓度范围宽、底物种类范围广,表明B3在PAEs的生物降解中具有良好的应用前景.With dibutyl phthalate（DBP）as the sole carbon source, we isolated a DBP-degrading strain; by its morphology as well as 16 S rDNA sequencing and phylogenetic analysis,the strain was identified as Stenotrophomonas sp.,designated as strain B3.Degradation of Phthalate esters（PAEs）by Stenotrophomonas was reported for the first time.The degradation efficiency of 0.05 g·L^（-1） DBP is 93.4%;B3 could efficiently degrade DBP up to 50 g·L^（-1）,and under the conditions of pH8,35 ℃,the degradation efficiency of 10 g·L^（-1） DBP by B3 reached 95.8%.Kinetic studies showed that DBP degradation by B3 followed first-order kinetics,and the initial degradation rate of 50 g·L^（-1） DBP can reach 1.25 g·L^（-1）·h-1.The degradation efficiencies of four other common PAEs（di-2-ethylhexylPhthalate、dimethyl phthalate、benzyl butyl phthalate、diethyl phthalate）as well as aniline,toluene and phthalic acid by B3 were 50% or higher,suggesting that B3 had a broad substrate spectrum.The ability of B3 to degrade various PAEs at high concentrations indicates that B3 is promising in bioremediation of PAEs.

关 键 词：寡养单胞菌 邻苯二甲酸酯 邻苯二甲酸二丁酯 生物降解 降解动力学 

分 类 号：X703[环境科学与工程—环境工程]