PRMT7与HSPA5相互作用促进肺癌转移的研究  

PRMT7 promotes lung cancer metastasis in a HSPA5 dependent manner

作　　者：程德志[1] 徐朝娜 张翔[1] 郑亮承[1] 张信波 谢德耀[1] CHENG De-zhi;XU Chao-na;ZHANG Xiang;ZHENG Liang-cheng;ZHANG Xin-bo;XIE De-yao(Department of Thoracic Cardiovascular,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,Chin)

机构地区：[1]温州医科大学附属第一医院胸外科,浙江温州325000

出　　处：《实用药物与临床》2018年第8期854-858,共5页Practical Pharmacy and Clinical Remedies

基　　金：浙江省自然科学基金(Y14H160162);温州市科技计划项目(Y20160049)

摘　　要：目的探究PRMT7在肺癌转移中的功能,并找到调控PRMT7的信号分子。方法通过免疫蛋白共沉淀法得到一系列与PRMT7相互作用的蛋白,通过蛋白质谱分析,结合生物信息学的方法,筛选具有显著意义的相互作用蛋白,并进行验证。结果确认了HSPA5等5个候选基因,经过对各种肺癌数据库的分析发现,候选基因的表达水平高于其他基因。结论 PRMT7与HSPA5存在相互作用,此发现有助于阐明PRMT7对肺癌细胞入侵和转移的促进作用。Objective To explore the role of PRMT7 in lung cancer metastasis and find out the signaling molecule which can regulate PRMT7. Methods A series of proteins in interaction with PRMT7 were obtained by coimmu- noprecipitation,and proteins of interaction with significance were screened out and tested by protein mass spectrometry and bioinformatics. Results Five candidate genes were identified. The analysis of various databases about lung cancer discovered that the expression level of candidate gene was higher than that of other genes. Conclusion PRMT7 can interact with HSPA5,which is helpful to clarify the promotion function of PRMT7 in the invasion and metastasis of lung cancer cell.

关 键 词：精氨酸甲基化 PRMT7 蛋白质结合 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]