烟酰胺N-甲基转移酶基因多态性与手球运动能力的关联性  

Nicotinamide N-methyl transferase gene polymorphism and its correlation with the athletic ability of handball players

作　　者：陈伟[1] 李江华[2] 顾建仁[1] 陈丰 夏华[1] 张彬[1] 焦启斌 郑凤 Chen Wei;Li Jiang-hua;Gu Jian-ren;Chen Feng;Xia Hua;Zhang Bin;Jiao Qi-bin;Zheng Feng(Suzhou Institute for Sports Science,Suzhou 215131,Jiangsu Province,China;Key Laboratory of Water Sports Training Monitoring and Intervention of General Administration of Sport of China,Sports College of Jiangxi Norme University,Nanchang 330022,Jiangxi Province,China;Suzhou Sports School,Suzhou 215131,Jiangst Province,China)

机构地区：[1]苏州市体育科学研究所,江苏省苏州市215131 [2]国家体育总局水上项目训练监控及干预重点实验室江西师范大学体育学院,江西省南昌市330022 [3]苏州市体育运动学校,江苏省苏州市215131

出　　处：《中国组织工程研究》2018年第28期4544-4549,共6页Chinese Journal of Tissue Engineering Research

基　　金：苏州市体育局体育科研局管课题(TY2017-203);苏州市体育科学研究所共同资助~~

摘　　要：背景:研究证实,烟酰胺N-甲基转移酶基因(nieotinamideN-methyl-transferase,NNMT)多态性与人体有氧运动能力相关。手球是一种混氧运动,比赛或平时训练中无氧运动占一定比例,主要进行有氧运动。目的:探讨NNMT基因单核苷酸多态性位点rs2256292与手球运动能力的关联。方法:采用PCR-LDR方法检测28名中国国家青年男子手球队运动员(实验组)和53名普通男学生(对照组)NNMT基因单核苷酸多态性位点rs2256292,测试相对最大摄氧量VO_(2 max)和骨骼肌百分比、体脂百分比身体成分指标。结果与结论:(1)实验组G等位基因和GG基因型频率显著高于对照组(P<0.05);(2)在28名运动员中,基因型GG组相对VO_(2 max)明显高于基因型CG组(P<0.05);在53名普通学生中,基因型GG组、基因型CG组及基因型CC组间相对VO_(2 max)无差异;(3)在28名运动员中,基因型GG组骨骼肌百分比高于基因型CC组、基因型CG组(P<0.05);在53名普通学生中,基因型GG组骨骼肌百分比高于基因型CG组(P<0.05);(4)在28名运动员中,基因型GG组、基因型CG组及基因型CC组间体脂百分比无差异;在53名普通学生中,基因型GG组、基因型CG组及基因型CC组间体脂百分比无差异;(5)结果表明,NNMT基因单核苷酸多态性位点rs2256292与手球运动能力明显相关,运动员更倾向于G等位基因,基因型GG携带者运动能力较强。BACKGROUND： Studies confirm that niacinamide N-methyl transferase gene（NNMT） polymorphism is associated with human body aerobic exercise capacity. Handball is a kind of mixed oxygen sports, accounting for a proportion of anaerobic exercise in competition or peacetime training, mainly in aerobic exercise. OBJECTIVE： To explore a NNMT gene single nucleotide polymorphism locus rs2256292 associated with the athletic ability of handball players. METHODS： The NNMT genetic polymorphism locus rs2256292 in 28 athletes from Chinese national male handball team（experimental group） and 53 male students（control group） was tested by polymerase chain reaction-ligase detection reaction. The relative VO（2max）and body constituents index of skeletal muscle, body fat percentage were also tested. RESULTS AND CONCLUSION： Frequencies of G allele and GG genotype in the experimental group were significantly higher than those in the control group（P〈0.05）. Among the 28 athletes, the relative VO（2max）in the GG genotype group was higher than that in the CG genotype group（P〈0.05）. Among the 53 general students, there were no differences in relative VO（2max）among genotypes. Among the 28 athletes, the skeletal muscle percentage in the GG genotype group was significantly higher than that in the CC and CG genotype groups（P〈0.05）. Among the 53 general students, the skeletal muscle percentage in the GG genotype group was significantly higher than that in the CG genotype group（P〈0.05）. In addition, there were no differences in the body fat percentage among genotypes in athletes or general students. These results suggest that the NNMT genetic polymorphism locus rs2256292 is significantly correlated with the athletic ability of handball players, with the athletes being more likely to have G allele and carriers of GG genotype having stronger athletic ability.

关 键 词：运动员 身体成分 多态性 单核苷酸 组织工程 手球 运动能力 组织构建 NNMT 基因多态性 相对最大摄氧量 骨骼肌百分比 体脂百分比 

分 类 号：R318[医药卫生—生物医学工程]