醒脑开窍针刺法对脑缺血再灌注损伤大鼠海马组织p-Akt(Ser473)表达及细胞凋亡的影响  

Effect of the Xingnao Kaiqiao acupuncture therapy on expression of p-Akt(Ser473) and neurocyte apoptosis in the hippocampus of rats with cerebral ischemia/reperfusion injury

作　　者：韩林[1] 高旸[1] 王旭慧[1] 张亚男[2] 张向宇[1] 孟丽娜[1] 朱倩[1] HAN Lin;GAO Yang;WANG Xuhui;ZHANG Yanan;ZHANG Xiangyu;MENG Lina;ZHU Qian(A cupuncture Department,The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Acupuncture Research Institute,The First Teaehing Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China)

机构地区：[1]天津中医药大学第一附属医院针灸部,天津300193 [2]天津中医药大学第一附属医院针灸研究所,天津300193

出　　处：《天津中医药》2018年第8期608-612,共5页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(81603688)

摘　　要：[目的]观察醒脑开窍针刺法对脑缺血再灌注损伤大鼠海马组织p-Akt(Ser473)表达及细胞凋亡的影响。[方法]将78只大鼠随机分为假手术组、模型组、电针组,每组26只。按Zea Longa线栓法复制大脑中动脉缺血再灌注模型(MCAO/R)。电针组给予针刺内关、人中、三阴交治疗,每日2次,持续治疗3 d。采用Zausinger 6分法评价神经功能,氯化三苯基四氮唑(TTC)染色检测脑梗死体积,苏木精-伊红(HE)染色观察海马组织病理损伤,流式细胞技术检测海马神经细胞凋亡率,Western Blot技术检测p-Akt(Ser473)、Bcl-2、Bax蛋白表达。[结果]电针组神经功能评分明显高于模型组(P<0.01),脑梗死体积明显小于模型组(P<0.01),海马组织病理损伤较模型组改善;电针组海马神经细胞总凋亡率明显小于模型组(P<0.05);电针组p-Akt蛋白、Bcl-2蛋白表达、Bcl-2/Bax比值较模型组均明显增大(P<0.05,P<0.01),Bax蛋白表达较模型组明显减小(P<0.01)。[结论]醒脑开窍针刺法可明显改善局灶性脑缺血再灌注大鼠的神经功能,减轻神经细胞凋亡,其作用可能通过激活PI3K/Akt信号通路起到脑保护效应。[Objective] To observe the effects of the Xingnao Kaiqiao acupuncture therapy on expressions of p Akt （Ser473） and neuroeyte apoptosis in the hippoeampus of rats with cerebral isehemia/repeffusion injury. [Methods] The 78 Sprague Dawley rats were randomly divided into three groups, the sham operation group, the I/R group and the eleetroaeupuneture group, 26 rats in each group. Tile cerebral isehemia repeffusion model was established By Longa＇ s suture method. In eleetroaeupuneture group, acupuncture was performed with Xingnao Kaiqiao method at main aeupoints of Neiguan （PC6）, Renzhong （DU26）, Sanyinjiao （SP6） and the electric acupuncture apparatus was connected to ipsilateral Neiguan （PC6） and Sanyinjiao （SP6）. Then aeupunetures were followed twice daily for 3 days. Neurological deficits were assessed By ethologie scoring according to Zausinger＇s neurologie assessment scales, the 2, 3, 4 triphenyltetrazolium chloride （TTC） staining was used to detect infarct volume, pathological brain damage was estimated by performing hematoxylin and eosin staining. In addition, neuroeyte apoptosis in the hippoeampus was detected by flow cytometry and tile protein expressions of p Akt （Ser473）, Bel 2 and Bax in hippoeampus were measured by Western blot. [Results] In comparison with the model group, the neurological deficits scores of tile eleetroaeupuneture group increased and tile infarction volume decreased obviously （P〈0.01）, the pathological damage alleviated significantly. At tile same time, compared with model group, tile hippoeampal neuron＇s total apoptosis rate obviously decreased （P〈0.05）in eleetroaeupuneture group accompanied with the increments of protein expressions of p Akt （Ser473）, Bel 2 and the ratio of Bel 2/Bax （P〈0.05, P〈0.01）, and with the reduction of protein expression of Bax （P〈0.01）. [Conclusion] Xingnao Kaiqiao acupuncture therapy can significantly improve neurological function and reduce neuroeyte apoptosis in rats with focal cerebral i

关 键 词：醒脑开窍针刺法 脑缺血再灌注 细胞凋亡 PI3K/AKT信号通路 

分 类 号：R743.31[医药卫生—神经病学与精神病学]