腺病毒生产工艺改进  

Improvement of the process for adenovirus production

作　　者：薛亮 王爱霞 归翔刚 XUE liang;WANG Aixiang;GUI Xianggang(Shanghai Sunway Biotech Co.,Ltd.,Shanghai 201206,China)

机构地区：[1]上海三维生物技术有限公司,上海201206

出　　处：《上海医药》2018年第15期72-74,78,共4页Shanghai Medical & Pharmaceutical Journal

摘　　要：在293细胞培养体系中感染腺病毒后,继续保持培养液中一定浓度的葡萄糖提供碳源,以维持宿主细胞更好的生命状态,促进腺病毒的复制、装配、释放,达到提高单批次病毒生产的产量。在病毒活性比稳定的前提下,改进后病毒颗粒数(产量)有41.1%的提升。After infection of adenovirus in the 293 cell culture, a certain concentration of glucose was continuously supplied in the culture medium to maintain the host cell in a better growth condition so as to promote the replication, assembly and release of the adenovirus and to improve the yield of single-batch virus production. The number of virus particles（production） could be improved by 41.1% on the premise of a stable virus activity ratio.

关 键 词：293细胞 腺病毒 残糖控制 

分 类 号：Q813.11[生物学—生物工程]