疏血通脉胶囊对脑缺血再灌注损伤模型大鼠的神经保护作用研究  

Study on Neuroprotective Effect of Shuxue Tongmai Capsules on Cerebral Ischemia-reperfusion Injury Model Rats

作　　者：陈朝[1] 刘泰[1] 黄一挚 黄敏[1] 甘典辉[1] 龚敏阳[1] 黎红丹 CHEN Chao;LIU Tai;HUANG Yizhi;HUANG Min;GAN Dianhui;GONG Minyang;LI Hongdan(TheFirst Affiliated Hospital of Guangxi University of TCM,Nanning 530023,China;Guangxi Health TechnicalVocational College,Nanning 530021,China)

机构地区：[1]广西中医药大学第一附属医院,南宁530023 [2]广西卫生职业技术学院,南宁530021

出　　处：《中国药房》2018年第16期2184-2188,共5页China Pharmacy

基　　金：广西自然科学基金资助项目(No.2014GXNSFBA118168);全国中药特色技术传承人才培训项目(No.国中医药人教函[2014]181号);广西卫生厅中医药医院制剂类课题(No.GZYZ-10-04)

摘　　要：目的:研究疏血通脉胶囊对脑缺血再灌注损伤模型大鼠的神经保护作用。方法:将36只SD大鼠随机分为假手术组(等体积0.9%氯化钠溶液)、模型组(等体积0.9%氯化钠溶液)、尼莫地平组(0.01 g/kg)和疏血通脉胶囊低、中、高剂量组(0.32、0.64、1.28 g/kg),每组6只。除假手术组外,其余5组大鼠均以线栓法建立脑缺血再灌注损伤模型,造模成功后灌胃相应药物,每天1次,连续28 d。于给药前及给药后3、7、14、28 d采用改良神经功能评分法进行大鼠神经功能缺损评分;给药后28 d以三磷酸脱氧鸟苷-生物素刻痕末端标记(TUNEL)法检测大鼠脑组织皮质区神经细胞凋亡情况,以免疫组化法检测大鼠脑组织皮质区脑源性神经营养因子(BDNF)表达情况。结果:与假手术组比较,模型组大鼠神经功能缺损评分显著升高,脑组织皮质区凋亡神经细胞数显著增加,BDNF表达显著增强,差异均有统计学意义(P<0.01)。与模型组比较,尼莫地平组和疏血通脉胶囊低、中、高剂量组大鼠给药后7、14、28 d神经功能缺损评分均显著降低;给药后28 d脑组织皮质区凋亡神经细胞数显著减少,BDNF表达显著增强,差异均有统计学意义(P<0.01)。结论:舒血通脉胶囊对脑缺血再灌注损伤模型大鼠的神经功能有一定保护作用,其机制可能与增强BDNF表达,抑制神经细胞凋亡有关。OBJECTIVE：To study the neuroprotective effect of Shuxue tongmai（SXTM） capsules on cerebral ischemiareperfusion injury model rats. METHODS：A total of 36 SD rats were randomly divided into sham operation group（equal volume 0.9% sodium chloride solution）,model group（equal volume 0.9% sodium chloride solution）,nimodipine group（0.01 g/kg）,SXTM capsules low-dose,medium-dose and high-dose groups（0.32,0.64,1.28 g/kg）,with 6 rats in each group. Except for sham operation group,cerebral ischemia reperfusion injury model of rats was established in other 5 groups,and relevant medicine was given intragastrically after modeling,once 2 day,for consecutive 28 d. Neurological function deficit scores were by m NSS method obtained before medication and 3,7,14,and 28 d after medication. The cortical neuron apoptosis in cortexarea of rats＇ braintissue was determined by TUNEL method 28 d after medication. The expression of brain-derived neurotrophic factor（BDNF）in cerebral tissue was determined by immunohistochemical staining mothed. RESULTS：Compared with sham operation group,the neurological function deficit score of model group was increased significantly 7,14,28 d after medication. The number of apoptotic cell was increased significantly 28 d after medication,and the expression of BDNF was strengthened significantly,there were significient difference（P〈0.01）. Compared with model group, neurological function scores of nimodipine group and SXTM capsules low-dose,medium-dose and high-dose groups were decreased significantly（P〈0.01）. The number of apoptotic cell was decreased significantly 28 d after medication（P〈0.01）,and the expression of BDNF was strengthened significantly,there were significient difference（P〈0.01）. CONCLUSIONS：SXTM capsules has a neuroprotective effect on cerebral ischemia-reperfusion injury model rats,the mechanism of which may be associated with inhibiting neuronal apoptosis and strengthening the expression of BDNF.

关 键 词：疏血通脉胶囊 脑缺血再灌注 脑源性神经营养因子 细胞凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学] R285[医药卫生—临床医学]