四川晒醋醋醅中一株产纤维素酶的芽孢杆菌分离筛选  

Screening of cellulose-producing Bacillus from Sichuan Sun vinegar

作　　者：刘军[1] 江若宇 王麒麟 帖余 王鑫 李丽[1] LIU Jun;JIANG Ruo-yu;WANG Qi-lin;TIE Yu;WANG Xin;LI Li(College of Biotechnology Engineering,Sichuan University of Science ＆ Enigneering,Zigong 643000,China)

机构地区：[1]四川理工学院生物工程学院,四川自贡643000

出　　处：《食品与发酵工业》2018年第6期140-144,共5页Food and Fermentation Industries

基　　金：固态发酵资源利用四川省重点实验室科项目(2015GTJ003);四川省教育厅项目(16ZA0253);四川省大学生创新创业训练计划项目(201510622063)

摘　　要：以四川晒醋醋醅为研究对象,从醋醅中筛选出1株产纤维素酶活较高的芽孢杆菌,并对其生理生化特性进行研究。通过平板划线和平皿生化反应对目的菌株进行分离纯化,通过形态特征以及16S rDNA同源序列分析对分离菌株进行鉴定。结果表明,筛选出的产纤维素酶活较高的菌株为苏云金芽孢杆菌(Bacillus thuringiensis),产纤维素酶活为161.33 u/g。Sichuan Sun vinegar was used as the research object,a strong cellulose production Bacillus strain was separated and its physiological-biochemical characteristics were studied. The separation and purification of strains were performed by plate streaking and plate biochemical reaction and the strain was identified through the morphological characteristics and was analyzed by 16 S rDNA sequence homology. The results showed that the strain with stronger cellulose-producing ability was Bacillus thuringiensis. Cellulase activity was 161. 33 u/g.

关 键 词：晒醋 芽孢杆菌 产纤维素酶 16S RDNA 

分 类 号：Q949.32[生物学—植物学]