固相萃取-气相色谱-火焰光度法同时测定植物性肽粉中42种有机磷农药残留  

Determination of 42 organophosphorus pesticide residues in plant-based peptide powder by SPE-GC-FPD

作　　者：安红梅[1] 柯润辉[1] 王丽娟[1] 杨春艳[1] 黄新望 田菲菲 尹建军[1] AN Hong-mei;KE Run-hui;WANG Li-juan;YANG Chun-yan;HUANG Xin-wang;TIAN Fei-fei;YIN Jian-jun(China National Research Institute of Food ＆ Fermentation Industries,Beijing 100015,China;Shimadzu Global COE,Shimadzu（China）Co.,Ltd.,Beijing 100020,China)

机构地区：[1]中国食品发酵工业研究院,北京100015 [2]岛津企业管理(中国)有限公司,北京100020

出　　处：《食品与发酵工业》2018年第6期245-250,共6页Food and Fermentation Industries

摘　　要：建立了植物性肽粉中42种有机磷农药的固相萃取-气相色谱-火焰光度检测器(SPE-GC-FPD)方法。样品用乙腈作为提取溶剂,提取液过石墨化炭黑-氨基复合柱净化,用乙腈-甲苯(体积分数为75%)洗脱,气相色谱-火焰光度检测器检测,外标法定量。在0.05和0.5 mg/kg的添加水平下,42种有机磷的平均回收率为72.8%~103.8%,相对标准偏差在2.44%~8.71%,该方法的检出限为0.005~0.020 mg/kg,定量低限为0.015~0.060 mg/kg。采用所建立方法测定了10种代表性的植物性肽粉中42种有机磷农药残留量,结果均未检出。该方法简单快捷、普及度高,适用于植物性肽粉中多种有机磷农药的分离与定量。A method for the determination of 42 kinds of organophosphorus pesticide in plant-based peptide powder by solid-phase extraction-gas chromatography-flame photometric detector（ SPE-GC-FPD） was established. The samples were extracted by acetonitrile,concentrated and purified by solid phase extracted on Pesti Carb/NH2 column,eluted with acetonitrile/toluene（ volume fraction is 75%）. Finally,the pesticides were determined by flame photometric detector（ FPD）,quantified by external standard method. The recoveries were 72. 8%-103. 8% at 2 spiked levels of 0. 05 and 0. 50 mg/kg for 42 organophosphorus pesticides,and the relative standard deviations（ RSDs） were between 2. 44%-8. 71%. The limits of detections were in the range of 0. 005-0. 020 mg/kg and the limits of quantitation were in the range of 0. 015-0. 060 mg/kg. A total of 10 plant-based peptides was tested and none of these pesticides were detected. The method can be applied to determine organophosphorus pesticides in plant-based peptide powder samples due to its efficiency and simplicity.

关 键 词：有机磷农药 植物性肽粉 固相萃取 气相色谱-火焰光度检测器(GC-FPD) 

分 类 号：TS225.21[轻工技术与工程—粮食、油脂及植物蛋白工程]