雷公藤对雌性大鼠性激素水平、卵巢内细胞凋亡以及骨代谢状况影响的联合观察与分析  

United observation and analysis of effect of tripterygium wilfordii on sex hormone levels,apoptosis in ovary and bone metabolism in female rats

作　　者：刘杰[1] 张茜 陈瑛[1] LIU Jie;ZHANG Qian;CHEN Ying(Rehabilitation Department,the Sixth Affiliated Hospital of Xinjiang Medical University,Urumchi 830001,China;Department of Respiratory Medicine,Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region,Urumchi 830001,China)

机构地区：[1]新疆医科大学第六附属医院康复科,新疆乌鲁木齐830002 [2]新疆维吾尔自治区中医医院呼吸科,新疆乌鲁木齐830000

出　　处：《海南医学院学报》2018年第14期1303-1306,共4页Journal of Hainan Medical University

基　　金：新疆医科大学科研创新基金项目(xydcx2016110)~~

摘　　要：目的:研究雷公藤对雌性大鼠性激素水平、卵巢内细胞凋亡以及骨代谢状况的影响。结果:选择成年雌性SD大鼠作为实验动物,并分为模型组和对照组,模型组通过雷公藤灌胃的方式建立卵巢早衰模型,对照组给予等剂量生理盐水灌胃。造模14d后取血清并测定性激素、骨代谢标志物的含量,取卵巢组织并测定凋亡基因的表达量,取胫骨组织并测定骨代谢标志基因的表达量。结果:模型组大鼠血清中FSH、LH、β-CTX、TRACP5b的含量显著高于对照组,E2、PINP、N-MID的含量显著低于对照组;模型组大鼠卵巢组织中Fas、FasL、APAF-1、Caspase-3的mRNA表达量显著高于对照组,Bcl-2、XIAP的mRNA表达量显著低于对照组;模型组大鼠骨组织中RUNX2、BAP、OPG的mRNA表达量显著低于对照组,RANKL、RANK的mRNA表达量显著高于对照组。结论:雷公藤所致卵巢早衰大鼠模型存在性激素改变、卵巢组织内细胞凋亡加剧以及全身骨代谢紊乱。Objective：To study the effect of Tripterygium wilfordii on sex hormone levels,apoptosis in ovary and bone metabolism in female rats.Methods：Adult female SD rats were chosen as experimental animals and divided into model group and control group,model group were made into premature ovarian failure models through intragastric administration of Tripterygium wilfordii,and control group were given intragastric administration of same dose of normal saline.Serum was collected 14 days after model making to determine the contents of sex hormones and bone metabolism markers,ovarian tissue was collected to determine the expression of apoptosis genes and tibial tissue was collected to determine the expression of bone metabolism marker genes.Results：FSH,LH,β-CTX and TRACP5 blevels in serum of model group were significantly higher than those of control group whereas E2,PINP and N-MID levels were significantly lower than those of control group（P〈0.05）;Fas,FasL,APAF-1 and Caspase-3 mRNA expression in ovarian tissue of model group were significantly higher than those of control group whereas Bcl-2 and XIAP mRNA expression were significantly lower than those of control group（P〈0.05）;RUNX2,BAP and OPG mRNA expression in bone tissue of model group were significantly lower than those of control group whereas RANKL and RANK mRNA expression were significantly higher than those of control group（P〈0.05）.Conclusion：There are sex hormone changes,apoptosis aggravation in ovarian tissue and systemic bone metabolism disorder in rat model with Tripterygium wilfordii-induced premature ovarian failure.

关 键 词：卵巢早衰 雷公藤 性激素 细胞凋亡 骨代谢 

分 类 号：R-332[医药卫生] R711.75