超滤法脱除猫爪草粗多糖中蛋白质的工艺研究  

Removal Protein of Ranunculus Ternatus Thunb Crude Polysaccharide by Ultrafiltration

作　　者：廖启元[1] 经嘉[1] 王晓阁[1] 刘东平[1] 刘丽敏[1] LIAO Qi-yuan;JING Jia;WANG Xiao-ge;LIU Dong-ping;LIU Li-min(Anhui College of Traditional Chinese Medicine, Wuhu, Anhui 24100)

机构地区：[1]安徽中医药高等专科学校,安徽芜湖241000

出　　处：《怀化学院学报》2018年第5期4-8,共5页Journal of Huaihua University

基　　金：安徽省教育厅重点项目"猫爪草多糖的提取分离及抗炎作用研究"(KJ2016A420)

摘　　要：研究了超滤法脱除猫爪草粗多糖中蛋白质的工艺.以多糖保留率和蛋白脱除率为评价指标,考察了截留分子量、温度、压力、多糖浓度、时间对超滤效果的影响.当选用截留分子量(MWCO)30 000、温度25℃、压力0.10 MPa、多糖的浓度3.0 mg/m L时,超滤处理100 min,多糖保留率和蛋白脱除率分别为78.2%和77.1%,优于Sevag法和TCA法.超滤法是一种可行的脱除猫爪草粗多糖中蛋白质的方法.Removal of protein of Ranunculus ternatus Thunb crude polysaccharide by ultrafiltration was studied. With the retention rate of polysaccharide and removal rate of protein as the index, molecular weight cut off（MWCO）,temperature, pressure, concentration of the polysaccharide and times were explored. The optimal condition were as follows：MWCO 30 000,45 ℃,0.10 MPa,3.0 mg/m L of the polysaccharide and 100 min,retention rate of polysaccharide and removal rate of protein were 78.2% and 77.1%,respectively,which was better than Sevag and TCA. It is feasible that removal of protein of Ranunculus ternatus Thunb crude polysaccharide by ultrafiltration.

关 键 词：猫爪草 多糖 脱蛋白 超滤 

分 类 号：R91[医药卫生—药学]