自拟促神经再生因子复合剂对大鼠脊髓损伤后凋亡相关蛋白bcl-2,bax,Caspase-3表达的影响  

Influence of self-devoloped neural regeneration promoting factor Compound(N6)(NRPFC) on the expression of the apoptosis related proteins bcl-2,bax and Caspase-3 in the spinal cord-injuried(SCI) rats

作　　者：祁健[1] 张俊江 赵廷宝 QI Jian,ZHANG Jun-jian,ZHAO Ting-bao.(Department of Spinal Cord Injury and Rehabilitation,the General Hospital of Jinan Military Region（former）,Jinan,Shandong 250031,China)

机构地区：[1]原济南军区总医院脊髓修复科,山东济南250031 [2]沂源县人民医院骨二科,山东淄博256100 [3]山东省肿瘤医院骨科,山东济南250031

出　　处：《实用医药杂志》2018年第7期627-632,共6页Practical Journal of Medicine & Pharmacy

基　　金：原济南军区总医院院长基金(2016ZX02)

摘　　要：目的观察促神经再生因子复合剂(N6)对脊髓损伤后不同时间点凋亡相关蛋白bcl-2,bax,Caspase-3表达变化的影响。方法应用Western blot和免疫组织化学染色方法 ,观察脊髓损伤(Spinal cord injury,SCI)后大鼠脊髓内凋亡相关蛋白bcl-2,bax,Caspase-3表达量变化及给予N6后对其表达变化的影响。结果在短时间内(8h)鞘内给予N6后,SCI模型大鼠脊髓内bax,Caspase-3表达量减少,bcl-2表达量增加,和16 h给药组相比差异具有统计学意义(P<0.05)。结论短时间内鞘内给予N6能够改变脊髓损伤后相关凋亡蛋白的表达。Objective To observe the expression of bcl-2,bax,Caspase-3 after injecting NRPFC（N6） at the different time points in spinal cord-injuried rats. Methods Using Western blot and immunohistochemical staining methods,authors observed the expression of bcl-2,bax,Caspase-3 after spinal cord injury and the expression change following intrathecaly injecting N6. Results At a short period of time（8 h） after intrathecal injecting N6,the level of bax,Caspase-3 was reduced compared with 16 h-injected groups. The level of bcl-2 was increased compared with 16 h-injected groups. Conclusion Intrathecal injection of N6 at a short period of time could change the expression of apoptosis related proteins following SCI.

关 键 词：脊髓损伤 促神经再生因子复合剂 bcl-2 bax Caspase-3 

分 类 号：R365[医药卫生—病理学]