miR-581对非小细胞肺癌A549细胞增殖、迁移和侵袭能力的影响  

Effect of miR-581 on the proliferation,migration and invasion ability of non-small cell lung cancer A549 cells

作　　者：全诗翠 夏荣木 胡佳 邓飞 Quan Shicui1,Xia Rongmu2,Hu Jia3,Deng Fei4(1. Department of Pathology, Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou 563099, China; 2. Department of Biochemistcy and Molecular Biology, Zunyi Medical University,Zunyi Guizhou 563099, China; 3. Key Laboratocy of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou 563099, China ;4. Department of Pathology, The First People＇s Hospital, Zunyi Guizhou 563000, China)

机构地区：[1]遵义医学院病理学教研室,贵州遵义563099 [2]遵义医学院生物化学与分子生物学教研室,贵州遵义563099 [3]遵义医学院附属医院贵州省细胞工程重点实验室,贵州遵义563099 [4]遵义市第一人民医院病理科,贵州遵义563000

出　　处：《遵义医学院学报》2018年第3期320-325,共6页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:81160300)

摘　　要：目的探讨miR-581对非小细胞肺癌A549细胞增殖,迁移和侵袭生物学行为的影响。方法转染miR-581以及miR-mimics进入A549细胞,未进行干扰的A549细胞为阴性对照组。流式细胞仪检测转染miR-581进入A549细胞的效率。CCK8法和克隆形成实验检测miR-581对肺癌A549细胞增殖能力的影响。划痕实验和Transwell迁移小室检测miR-581对肺癌A549细胞迁移和侵袭能力的影响。结果转染miR-581进入A549细胞的效率为(75.3±3.8)%。转染miR-581进入A549细胞后,同一时间细胞活力的检测值相对于未进行干扰的A549细胞明显降低,差异具有统计学意义(P<0.05)。转染miR-mimics的细胞增殖曲线与未进行干扰的A549细胞无明显差异,同一时间细胞活力的检测值相对于未进行干扰的A549细胞,差异无统计学意义(P>0.05)。克隆形成实验结果证实miR-581能够显著抑制A549细胞的克隆形成能力。Transwell迁移小室检测和划痕实验结果显示miR-581能够显著抑制A549细胞的迁移和侵袭能力。结论miR-581能够抑制非小细胞肺癌A549细胞增殖,同时能够抑制其迁移和侵袭能力,提示miR-581可能作为一种潜在的肺癌治疗手段。Objective To investigate the effects of miR-581 on biological behavior of non-small cell lung cancer A549 cell line including proliferation,migration and invasion. Methods MiR-581 and miR-mimics were transfected into A549 cells using RNAi Max and normal cultured cells were used as negative control. The ability of RNAi Max to transfect miR-581 into A549 cells was detected by flow cytometry. The cell proliferation activity was measured by CCK8 assay,and the clone formation assay was used to explore the effect of miR-581 on the proliferation of A549 cells. The effect of miR-581 on the migration and invasion ability of lung cancer A549 cells was examined by scratch test,Transwell migration chamber and Transwell migration chamber pre-plated with Matrigel. Results The efficiency of RNAi Max transfected into A549 cells with miR-581 was（ 75. 3 ± 3. 8）%. After transfected with miR-581 into A549 cells,the cell proliferation activity changed. Compared with the normal cells,the viability was significantly decreased at the same time（ P〈0. 05）. The proliferation curve of miR-mimics transfected cells showed no significant difference compared with that of normal cells. There was no significant difference in cell viability between the two groups at the same time（ P〈0. 05）. The result of clone formation assay confirmed that miR-581 can significantly inhibit the colony-forming ability of A549 cells,and miR-mimics did not. Transwell migration chamber detection and scratch test results show that miR-581 can significantly inhibit the migration and invasion of A549 cells,miR-mimics no such effect. Conclusion MiR-581 can not only inhibit the proliferation,but also the migration and invasion ability of non-small cell lung cancer A549 cells,suggesting miR-581 may serve as a potential therapeutic approach for lung cancer. However,the specific mechanism is worth further exploration.

关 键 词：miR-581 A549细胞 增殖 迁移 侵袭 

分 类 号：R73-36[医药卫生—肿瘤]