无血预充大鼠体外循环模型的建立  

The establishement of an extracorporeal circulation rat model with exsanguine priming

作　　者：李健 郭震 路丽明[3] 李欣 Li Jian;Guo Zhen;Lu Liming;Li Xin(Department of Cardiovascular Surgery,Shanghai Chest Hospital,Shanghai Jiao Tong University,Shanghai 200030,China)

机构地区：[1]上海交通大学附属胸科医院心外科,上海200030 [2]上海交通大学附属胸科医院体外循环室,上海200030 [3]上海交通大学医学院上海市免疫研究所,上海200030 [4]复旦大学附属中山医院心外科,上海200030

出　　处：《中国体外循环杂志》2018年第3期184-188,共5页Chinese Journal of Extracorporeal Circulation

基　　金：上海市科学技术委员会医学引导类科研计划项目(17411971300)

摘　　要：目的建立稳定可重复的无血预充大鼠体外循环模型。方法 15只成年雄性SD大鼠,水合氯醛腹腔麻醉,14 G套管针行气管插管,呼吸机维持通气;自制前端带侧孔16 G套管针穿刺右颈外静脉置管作静脉引流,20 G、24 G、24 G套管针分别穿刺右颈总动脉、左股动脉、左股静脉置管作动脉灌注、血压监测、静脉补液。采用无血预充,自体血液经小动物膜式氧合器进行气体交换,维持体外循环60 min。监测体外循环开始前、过程中、结束后的血流动力学并进行血气分析。结果连续15只大鼠建立模型过程中,头3只外科操作失败死亡,后12只大鼠体外循环模型成功建立,体外循环中及停机后生命体征稳定,存活时间超过1天。结论成功建立无血预充大鼠体外循环微创模型,为进一步探究体外循环相关损伤机制和保护措施提供了可靠动物平台。Objective To establish an extracorporeal circulation（ ECC） rat model with the advantage of stabilization and repeatability. Methods Fifteen adult male Sprague-Dawley rats received anesthesia induction with chloral hydrate by intraperitoneal administration and maintained mechanicl ventilation by transoral endotracheal intubation using a 14 G catheter. A homemade multi lateral orifice 16 G catheter was cannulated in the right jugular vein for venous drainage. The right carotid artery,left femoral artery and left femoral vein were respectively cannulated for arterial inflow,blood pressure monitoring and fluid infusion,by 20 G,24 G,24 G catheters. The circuits were primed without blood,the autologous blood was oxygenated in the small animal special purposed oxygenator. The ECC lasted for 60 minutes. Hemodynamic parameters and blood analysis were monitored before ECC,during ECC and after ECC. Results The first 3 of 15 rats died during the progress of establishement of ECC,the rest 12 rats survived more than 1 day,whose hemodynamic parameters and blood analysis in different time points were in accord with normal standard. Vital signs of the 12 rats were stable after the termination of ECC. Conclusion The ECC rat model with exsanguine priming was successfully established.

关 键 词：体外循环 动物模型 大鼠 

分 类 号：R-332[医药卫生] R654.1