LI-cadherin在进展期胃癌中的表达及对人胃癌细胞株MKN28的作用及其机制  

Expression of LI-cadherin in advanced gastric cancer and its effect and mechanism on human gastric cancer cell line MKN28

作　　者：林晶[1] 江小杰[2] 蔡清河[2] 陈智伟[1] LIN Jing;JIANG Xiao-jie;CAI Qing-he;CHEN Zhi-wei(Department of Pathology;Department of Hepatobiliary Surgery,the Affiliated Hospital of Putian University,Putian 351100,China)

机构地区：[1]福建省莆田学院附属医院病理科,莆田351100 [2]福建省莆田学院附属医院肝胆外科,莆田351100

出　　处：《临床与实验病理学杂志》2018年第6期595-600,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：福建省中青年教师教育科研项目(A类)(JA13289);福建省卫生厅青年科研课题(2012-2-145);福建省自然科学基金(2017J0105)

摘　　要：目的探讨肝肠钙黏连蛋白(liver-intestine cadherin,LI-cadherin)在进展期胃癌中的表达和对人胃癌细胞株MKN28的作用及机制。方法采用免疫组化法检测LI-cadherin蛋白在进展期胃癌中的表达;CCK8、克隆实验、Transwell、流式细胞术检测LI-cadherin基因干扰后MKN28细胞的生物学行为变化情况;双荧光素酶检测系统及Western blot法检测干扰LI-cadherin后对NF-κB活性的影响。结果 LI-cadherin在慢性胃炎中不表达,在中+高分化进展期胃癌中表达升高,在低分化进展期胃癌中表达降低;干扰LI-cadherin后,MKN28细胞增殖能力、克隆形成数目及侵袭能力明显受到抑制(P<0.05);流式细胞术检测结果显示,干扰LI-cadherin后导致G_1期细胞比例显著增加,而S期细胞比例显著降低(P<0.05);干扰LI-cadherin后,NF-κB信号的活性降低,核易位减少。结论 LI-cadherin蛋白在中+高分化进展期胃癌组织表达明显升高。干扰LI-cadherin表达后,MKN28细胞的恶性生物学行为明显减低。其机制可能与NF-κB信号通路相关。Purpose To investigate the expression of LIcadherin in advanced gastric cancer and its effect and mechanism on human gastric cancer cell line MKN28.Methods Immunohistochemical staining was performed on advanced gastric cancer to examine the LI-cadherin protein expression.CCK8,colony formation assay,transwell assay,flow cytometry were used to detect the changes in the biological behavior of MKN28 cells after LI-cadherin gene interference.Dual-luciferase reporter assay and Western blot were used to detect the activity of the NF-κB after the interference of LI-cadherin gene.Results LIcadherin in chronic gastritis was negative,in well-and moderately-differentiated advanced gastric cancer was significantly increased,in poorly-differentiated was decreased.Cell proliferation,colony formation,and invation ability were inhibited in the group of knockdown expression of LI-cadherin（P〈0.05）.Flow cytometry results showed that knockdown expression of LIcadherin resulted in a significant increase in the proportion of cells in G1 phase,while the proportion of cells in S phase was significantly lower（P〈0.05）.Knockdown expression of LIcadherin resulted in the decreased activation of NF-κB and restricted its translation.Conclusion LI-cadherin in well-and moderately-differentiated advanced gastric cancer is significantly increased.Knockdown of LI-cadherin can strongly decrease the malignant biological characteristics of human gastric cancer cell line MKN28.Its mechanism may be associated with the NF-κB pathway.

关 键 词：胃肿瘤 进展期胃癌 LI-CADHERIN 机制 

分 类 号：R735.2[医药卫生—肿瘤]