猫中脑髓外压迫性损伤模型的建立  

Establishment of midbrain injury model using intracranial water-sac compression

作　　者：何乐 王红云 陈一元 李储忠 HE Yue, WANG Hong-yun, CHEN Yi-yuan, et al.(Beijing Neurosurgical Institute, Beijing 100050, China)

机构地区：[1]北京市神经外科研究所,北京100050

出　　处：《临床和实验医学杂志》2018年第14期1476-1479,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨建立中脑压迫性损伤实验动物模型的方法和意义。方法采用84只普通家猫,体重2.5~4.0 kg,全麻下开颅在中脑背侧置入微型硅胶水囊,造成中脑髓外压迫性损伤,根据不同压迫容量(0.25 ml、0.50 ml、1.00 ml、1.50 ml和2.00 ml)和不同压迫时间(2 h、12 h、24 h和48 h)分组,观察损伤后动物意识、呼吸、运动功能、瞳孔及神经反射情况,同时记录脑干听觉诱发电位(BAEP)变化,评估动物神经系统功能损伤程度,评价模型的稳定性。结果硅胶水囊置入猫中脑背侧后,可以造成可控的中脑损伤,并引起相应的病理生理改变,包括意识水平下降、运动障碍和神经反射异常等,压迫容量和持续时间与中脑损伤程度呈正相关。同时,损伤动物BAEP波潜伏期明显延长,与脑干BAEP的异常程度明显相关。结论应用硅胶水囊置入猫中脑背侧,建立中脑损伤的实验动物模型稳定、可控,可以应用于中脑损伤的实验研究。Objective To explore the methods and its significance of midbrain compression injury experimental animal model. Methods Eighty-four domestic cats weighed between 2.5 and 4 kg were selected and a micro-silica-water-sacat was implanted at dorsal midbrain with different volume （0.25 ml, 0.50 ml, 1.00 ml, 1.50 ml and 2.00 ml） or different duration （2 h, 12 h, 24 h and 48 h） of compression to form the midbrain injury. Observing the functional changes of the nervous system which was injured by the compression including consciousness, respiration, motor function, pupillary reflex and other related nerve reflex. At the same time, BAEP changes were recorded to assess neurological impairment. Results Implanting the micro-silica-water-sac at dorsal midbrain could injure the midbrain and cause the corresponding pathophysiological performance. The compression volume and duration were positively associated with the damaged degree and also significantly related to the changes of BAEP. Conclusion The experimental animal model of midbrain injury established by implanting intracranial micro-silica-water-sac is stable and controllable, and it can be applied to the experimental study of midbrain injury.

关 键 词：猫 中脑损伤 动物模型 

分 类 号：R-332[医药卫生] R651.15