靶向IRF-2基因的siRNA对胰腺腺泡细胞凋亡的机制研究  

The effect of IRF-2 gene siRNA on the apoptosis of pancreatic acinar cells

作　　者：李艳[1] 谢东方[1] 邵珂 徐鹏[1] LI Yan, XIE Dong-fang, SHAO Ke, et al.(Department of Emergency, Qingdao University Second Clinical Medical College, Qingdao City Central Hospital, Qingdao Shandong 266033, China)

机构地区：[1]青岛大学第二临床医学院(青岛市中心医院)急诊内科,山东青岛266033

出　　处：《临床和实验医学杂志》2018年第14期1512-1515,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨靶向干扰素调节因子2(IRF-2)基因的小干扰RNA(siRNA)对胰腺腺泡细胞凋亡的影响及机制。方法以脂质体Lipofectamine TM2000为载体,参照其转染说明将设计并合成的IRF-2的siRNA转染AR42J细胞,并设置阴性对照siRNA(NC)及空白组(仅加入脂质体)。IRF-2的siRNA及阴性对照siRNA转染AR42J细胞24 h后与雨蛙肽(10-8mol/L)同培养,流式细胞仪检测各组细胞凋亡率及活性氧族(ROS)含量;Western blotting检测Bcl-2、Bax和p53的蛋白表达。结果转染IRF-2的siRNA的AR42J细胞IRF-2蛋白表达降低,与空白组比较差异具有统计学意义(P<0.05)。雨蛙肽可明显诱导AR42J细胞凋亡,诱导ROS产生,上调Bax和p53蛋白表达,下调Bcl-2的蛋白表达,与NC组比较差异具有统计学意义(P<0.05),而转染si-IRF-2后可降低由雨蛙肽诱导的AR42J细胞凋亡和ROS产生,下调Bax和p53蛋白表达,上调Bcl-2的蛋白表达,与NC+雨蛙肽组比较差异具有统计学意义(P<0.05)。结论靶向IRF-2基因的siRNA可抑制胰腺腺泡细胞凋亡,机制可能与降低细胞内ROS含量及下调Bax、p53表达和上调Bcl-2表达有关。这种抑制可能与增加急性胰腺炎的炎症程度有关。Objective To investigate the effect of IRF-2 gene siRNA on the apoptosis of pancreatic acinar cells. Methods Lipofectamine TM2000 liposome as a carrier, IRF-2 siRNA was transfected to AR42J cells which were designed and synthesized refer to the description of transfection, negative control siRNA （NC） and blank group were set up, IRF-2 siRNA and negative control siRNA were co cultured with Caerulein （10 -8 mol/L） after siRNA were transfected into AR42J cells for 24 h. The cell apoptosis rate and ROS content were detected in each group by flow cytometry; expression of Bax, Bcl-2 and p53 protein were detected by Western blotting. Results The protein expression of IRF-2 in AR42J cells transfected with IRF-2 siRNA was reduced, and the difference was statistically significant compared with the blank group （ P 〈0.05）. Caerulein significantly induced apoptosis in AR42J cells, induced ROS production, up-regulated the expression of Bax and p53 protein, down regulated the protein expression of Bcl-2, the difference was statistically significant compared with the NC group （ P 〈0.05）. After transfecting si-IRF-2 can decrease apoptosis rate and ROS content of AR42J cells induced by cerulein, down regulated the expression of Bax and p53 protein, up-regulated the expression of Bcl-2 protein, the difference was statistically significant compared with NC＋Caerulein group （ P 〈0.05）. Conclusion siRNA targeting IRF-2 gene can inhibit the apoptosis of pancreatic acinar cells, which may be related to the reduction of intracellular ROS content, down regulation of Bax and p53 expression and up regulation of Bcl-2 expression. This inhibition may be associated with an increase in the degree of inflammation of AP.

关 键 词：胰腺腺泡细胞 IRF-2基因 凋亡 

分 类 号：R576[医药卫生—消化系统]