复方苁蓉颗粒总黄酮提取工艺优化及其体外抗氧化活性  

Extraction optimization for total flavonoids from Compound Congrong Granules and the in vitro antioxidant activity

作　　者：高建德[1] 宋开蓉 朱晓玉[1] 刘雄[1] 陈晖[1] GAO Jian-de;SONG Kai-rong;ZHU Xiao-yu;LIU Xiong;CHEN Hui(Gansu University of Chinese Medicine,Lanzhou 730000,Chin)

机构地区：[1]甘肃中医药大学,甘肃兰州730000

出　　处：《中成药》2018年第7期1496-1500,共5页Chinese Traditional Patent Medicine

基　　金：甘肃省中医药管理局科研项目(GZK-2015-27);甘肃省中医方药挖掘与创新转化重点实验室开放基金项目(ZYFYZH-KJ-2015-002);甘肃省科技计划项目(1308RJZA207)

摘　　要：目的优化复方苁蓉颗粒总黄酮提取工艺,并评价其体外抗氧化活性。方法在单因素试验基础上,以乙醇体积分数、料液比、提取时间为影响因素,总黄酮提取率为评价指标,响应面法优化提取工艺。然后,测定总黄酮对DPPH·、·OH自由基的清除作用。结果最佳条件为乙醇体积分数65%,料液比1∶10,提取时间2.5 h,总黄酮提取率20.93%。总黄酮对2种自由基有明显的清除作用,并呈量效关系。结论该方法稳定可靠,可用于提取具有显著体外抗氧化活性的复方苁蓉颗粒总黄酮。AIM To optimize the extraction for total flavonoids from Compound Congrong Granules and to evaluate the in vitro antioxidant activity. METHODS With ethanol concentration,solid-liquid ratio and extraction time as influencing factors,extraction rate of total flavonoids as an evaluation index,response surface method was applied to optimizing the extraction on the basis of single factor test. And the scavenging effects of total flavonoids on DPPH·,·OH free radicals were determined. RESULTS The optimal conditions were determined to be 65%for ethanol concentration,1 ∶ 10 for solid-liquid ratio,and 2. 5 h for extraction time,total flavonoids demonstrated the extraction rate of 20. 93%. The total flavonoids exhibited obvious scavenging effects on two free radicals in a dose-dependent manner. CONCLUSION This stable and reliable method can be used for the extraction for total flavonoids with significant in vitro antioxidant activity from Compound Congrong Granules.

关 键 词：复方苁蓉颗粒 总黄酮 提取 体外抗氧化活性 响应面法 

分 类 号：R284.2[医药卫生—中药学]